The Effect of Co-administration of Aloe vera Gel and Cinnamon zeynalicum Hydroethanolic Extract on Wound Healing Process in Diabetic Mice

Objective-This study was conducted to evaluate the effect of co-administration of Aloe vera gel and cinnamon zeynalicum bark hydroethanolic extract on process of wound healing in diabetic mice model.Design- Experimental Study.Animals- Seventy-two male BALB/c mice.Procedures- A single full-thickness excisional wound was created on back of each mouse with 7-mm punch biopsy. Animals were divided into four groups including control, 5% Aloe vera gel (A.vera), 5% cinnamon extract (C. zeynalicum) and combination of 5% Aloe vera gel + 5% cinnamon extract (C. zeynalicum + A. vera). The rate of wound closure, histological assessment, hydroxyproline content and biochemical evaluation for total antioxidant capacity (TAC), superoxide dismutase (SOD) and malondialdehyde (MDA) were done at 3, 7 and 14 day after wound creation.Results-The rate of wound closure, number of fibroblast, collagen deposition, epithelium thickness and tissue hydroxyproline, TAC and SOD content were significantly enhanced in treated animals in comparison to control group (P<0.05). Moreover, tissue edema, immune cells infiltration and MDA content were significantly decreased in treated animals versus control group (P<0.05).Conclusion and Clinical Relevance- Topical co-administration of Aloe vera gel and cinnamon zeynalicum bark hydroethanolic extract have synergistic interaction effect and improved wound healing in diabetic rat and could be recommended as a new topical herbal drug production for treating of the diabetic wound.