Protective role of glutathione in buck semen cryopreservation

نویسندگان

  • A. Towhidi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
  • M. Emamverdi Ph.D. Student in Animal Physiology, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
  • M. Khodaei Motlagh Department of Animal Science, Faculty of Agriculture, Arak University, Arak, Iran
  • M. Shakeri Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
  • M. Sharafi Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
  • M. Zhandi Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
  • R. Noei Razliqi Graduated from College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده مقاله:

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five equal parts and each part was diluted by one of the above mentioned groups. After freeze-thawing process, motility and velocity, plasma membrane integrity and functionality, and apoptosis features of spermatozoa were evaluated. The results of this experiment showed that total motility (50.75 ± 2.33), plasma membrane integrity (55.75 ± 3.01) and functionality (46.75 ± 2.79) were higher in LG-1 extender compared to other extenders (P

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Protective role of glutathione in buck semen cryopreservation.

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

متن کامل

protective role of glutathione in buck semen cryopreservation

the aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. in this experiment, different concentrations of glutathione [0 (lg-0), 0.5 (lg-0.5), 1 (lg-1), 1.5 (lg-1.5), and 2 (lg-2) mm] were added in a soybean lecithin-based extender. a total of 16 ejaculates from four bucks were collected and pooled. each pooled sample was divided into five...

متن کامل

Advances in Boar Semen Cryopreservation

The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI...

متن کامل

Advances in avian semen cryopreservation

Semen cryopreservation in domestic birds has been studied extensively in the past fifty years. However, efficient methods to freeze semen of chicken of different breeds have emerged only in the last decade of the 20 century. Methods using cryoprotectants Glycerol, Dimethyl Acetamide and Dimethyl sulfoxide, slow or rapid freezing-thawing procedures, pellets or ampoules or straws packaging have b...

متن کامل

Cyclophosphamide-Induced Lipid Peroxidation and Changes in Cholesterol Content: Protective Role of Reduced Glutathione

       The study was designed with an aim to evaluate the protective effects of reduced glutathione on cyclophosphamide induced lipid peroxidation and also changes in cholesterol content. Goat liver and white New Zealand rabbit were used as lipid source for the models. Lipid peroxidation study was performed by measuring the malondial...

متن کامل

Effect of Different Levels of Egg Yolk on Cryopreservation of Black Bengal Buck Semen in Tris Egg yolk Citrate Fructose Glycerol Extender

This study was conducted to assess the physical and morphological characteristics of fresh, pre-freeze and post-thaw black Bengal buck (Capra hircus) semen processed in Tris-egg yolk-citrate-fructose-glycerol (TEYCFG) extender containing 4 different concentrations (2.5, 5, 7.5 and 10%) of egg-yolk. Ejaculates were collected once a week for eight weeks from six mature bucks (48 ejaculates) using...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 16  شماره 3

صفحات  298- 300

تاریخ انتشار 2015-09-20

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023