P-36: Administration of L-Carnitine and Homogenized Testis Tissue on The Structure of Testis and Sperm Parameter in Busulfan-Treated Rats

Background: Busulfan is a chemotherapy drug that is widely used for cancer treatment. However, administration of busulfan to male patients with malignant diseases may cause temporary or permanent sterility. Therefore, research into drugs or Materials to reduce the side effects of busulfan on the male reproductive system is necessary. In the present study the protective effects of L-carnitine and testis-homogenized tissue on the reproductive system of male adult rat following busulfan treatment has been investigated. Materials and Methods: Stereological technique was used for estimation volumes of testis, seminiferous tubules and interstitial tissue, the length and diameter of seminiferous tubules, germinal epithelium height, length of flagella and total number of some spermatoginic cell lines. Also Sperm Analysis Techniques was used for assessment of sperm parameters. Results: Based on this research, the length of sperm flagella and sperm count significantly increased in animals treated with L-carnitine Or Testis homogenized tissue in comparison with the busulfan-treated group. Testis homogenized tissue increased testis volume (6.5%) and weight (8.6%), tubule and interstitial tissue volume (6.9% and 11.7% respectively), seminiferous tubule diameter (3.8%), germinal epithelium height (13%) and sperm count (7.5%), and decreased sperm with abnormal morphology (1%) in comparison with the Lcarnitine+ busulfan treated group Conclusion: It seems the use of L-carnitine and testis- homogenized tissue with busulfan decreases some side effects of this drug on the male reproductive system. However, in our study, testis-homogenized tissue is more effective than L-carnitine and led to the recovery of both testis and sperm parameters after treatment with busulfan.