In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant
Authors
Abstract:
To optimize conditions for micropropagating Galanthus transcaucasicus Fomin, the effect of explant type, different concentrations of sucrose, type and different concentrations of auxin were examined on bulblet production. For the induction of bulblets, three types of bulb scales were usesd as explants. A two-step sterilization procedure was applied. Assessments were made after incubation for 20 weeks at 23±1ºC with 16h photoperiods. Highest number of bulblets were proliferated culturing bulb scales on modified MS medium supplemented with 2.0 mg.l-1 BA and 2.0 mg.l-1 IBA. About 27% of bulblets were bigger than 5 mm in diameter, suitable for acclimatization. With the increase of sucrose concentration, number of bulblets decreased, but their size increased. This protocol provided a basis for future study on large-scale multiplication system for commercial nurseries of Galanthus transcaucasicus.
similar resources
The first phytochemical report of Galanthus transcaucasicus Fomin
UNLABELLED BACKGROUND AND THE PURPOSE OF STUDY: Galanthus transcaucasicus Fomin (Amaryllidaceae) is an endemic species to the Caucasia and Alborz mountains in Iran which locally named "Gole-Barfi". While there are many reports on pharmacological activities of Galanthus species' alkaloids, there is no report on G. transcaucasicus and this article is the first phytochemical study on this species....
full textNerinine and homolycorine, amaryllidaceae alkaloids from the bulbs of Galanthus transcaucasicus Fomin
Background and objectives: Many members of the Amaryllidaceae are regarded as toxic. The toxic constituents that occur in the whole family are referred to as the Amaryllidaceae alkaloids. The main aim of this study was the identification of alkaloid compounds from Galanthus transcaucasicus Fomin, a medicinal plant from Amaryllidaceae. Methods: Planar and column chromatography techniques were us...
full textIn Vitro Propagation of "critically Endangered" Medicinal Plant-asparagus Racemosus
1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...
full textNerinine and homolycorine, amaryllidaceae alkaloids from the bulbs of Galanthus transcaucasicus Fomin
Background and objectives: Many members of the Amaryllidaceae are regarded as toxic. The toxic constituents that occur in the whole family are referred to as the Amaryllidaceae alkaloids. The main aim of this study was the identification of alkaloid compounds from Galanthus transcaucasicus Fomin, a medicinal plant from Amaryllidaceae. Methods: Planar a...
full textDifferent modes of plant regeneration and factors affecting in vitro bulblet production in Ornithogalum virens
Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant. Murashige and Skoog’s (MS) medium containing 1 mg/L (5.4mM) NAA and 2 mg/L (4.4mM) BA was most effective in direct induction of shoot buds from explant. Callus cultures were raised from the bulb ...
full textIn Vitro Production and Efficient Quantification of Major Phytopharmaceuticals in an Endangered Medicinal Herb, Swertiachirata
Swertiachirata is an endangered medicinal herb with a wide range of pharmacological activities, particularly as an anti-diabetic in an herbal formulation Diabecon (Himalaya®). The endangered status has resulted in short supply of plant material, thereby, resulting in adulteration practices. It is, therefore, of paramount importance to develop a shoot biomass production system enriched with majo...
full textMy Resources
Journal title
volume 6 issue 2
pages 1- 8
publication date 2022-12-22
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023