diagnostic value of lectins in differentiation of molar placentas

Authors

fatemeh atabaki pasdar department of anatomy and cell biology, mashhad university of medical science, mashhad, iran

alireza khooei department of pathology, imam reza hospital, mashhad university of medical sciences, mashhad, iran

alireza fazel department of anatomy and cell biology, mashhad university of medical sciences, mashhad, iran

mahmoud mahmoudi immunology research centre, buali research institue, mashhad university of medical sciences, mashhad, iran

abstract

objective(s)distinction of hydatidiform moles from non-molar specimens and subclassification of hydatidiform moles as complete and partial are important for clinical practice, but diagnosis based solely on histomorphology suffers from poor interobserver reproducibility. nowadays, pathologists rely on molecular techniques, however these methods are technically difficult, relatively expensive, and time consuming, and cannot be applied in all laboratories. therefore, a relatively easy, time- and cost-effective ancillary tool, would be helpful. this study aimed to assess the role of lectins in differential diagnosis of molar placentas.materials and methodslectin histochemistry with a panel of hrp-conjugated lectins comprising sba, dba, mpa, pna, vva, uea-1, lta, gs-і (b4), and wga were performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (partial and complete moles), formalin-fixed paraffin-embedded tissue samples. on the basis of staining intensity, sections were graded and kruskal-wallis non-parametric statistical test was used to compare differences between samples.resultsthere was a significant difference between the reactivities of lta and uea-і with syncytiotrophoblasts of molar and non-molar specimens (p<0.001). these lectins generally showed a moderate reactivity with syncytiotrophoblasts of molar group but did not react with this cell population in non-molar group. furthermore, wga showed relatively increased reaction with syncytiotrophoblasts of molar tissues compared with abortions, however, this did not reach to statistical significance (p=0.07). no major differences were seen in other lectins reactivities between the studied groups.conclusionthe present study showed that uea-1 and lta lectins may be used as cytochemical probes in differentiating molar from non-molar placentas, but did not differentiate partial moles from complete moles.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Diagnostic Value of Lectins in Differentiation of Molar Placentas

Objective(s)Distinction of hydatidiform moles from non-molar specimens and subclassification of hydatidiform moles as complete and partial are important for clinical practice, but diagnosis based solely on histomorphology suffers from poor interobserver reproducibility. Nowadays, pathologists rely on molecular techniques, however these methods are technically difficult, relatively expensive, an...

full text

Diagnostic Value of Lectins in Differentiation of Molar Placentas

OBJECTIVES Distinction of hydatidiform moles from non-molar specimens and subclassification of hydatidiform moles as complete and partial are important for clinical practice, but diagnosis based solely on histomorphology suffers from poor interobserver reproducibility. Nowadays, pathologists rely on molecular techniques, however these methods are technically difficult, relatively expensive, and...

full text

The Diagnostic Value of B-Mode Sonography in Differentiation of Malignant and Benign Tumors of the Parotid Gland

Introduction: Different imaging modalities are used to evaluate salivary gland diseases, including tumors. Ultrasonography (US) is the preferred method on account of its ease of use, affordability, safety profile, and good tolerance among patients. The aim of this study was to evaluate the role of US in differentiating malignant from benign parotid tumors, in the context of previous controversy...

full text

high volatility, thick tails and extreme value theory in value at risk estimation: the case of liability insurance in iran insurance company

در این بررسی ابتدا به بررسی ماهیت توزیع خسارات پرداخته میشود و از روش نظریه مقادیر نهایی برای بدست آوردن برآورد ارزش در معرض خطر برای خسارات روزانه بیمه مسئولیت شرکت بیمه ایران استفاده میشود. سپس کارایی نظریه مقدار نهایی در برآورد ارزش در معرض خطر با کارایی سایر روشهای واریانس ، کواریانس و روش شبیه سازی تاریخی مورد مقایسه قرار میگیرد. نتایج این بررسی نشان میدهند که توزیع ،garch شناخته شده مدل...

15 صفحه اول

faculty of psychology and social sciences group of anthropology master thesis in major of anthropology

چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...

ideological and cultural orientations in translation of narrative text: the case of hajji baba of isfahan

در میان عواملی که ممکن است ذهن مترجم را هنگام ترجمه تحت تأثیر قرار دهند، می توان به مقوله انتقال ایدئولوژی از طریق متن یا گفتمان اشاره کرد. هدف از این تحقیق تجزیه و تحلیل جنبه های ایدئولوژیکی و فرهنگی متن مبدأ انگلیسی نوشته جیمز موریه تحت عنوان سرگذشت حاجی بابای اصفهانی ( 1823) و ترجمه فارسی میرزا حبیب اصفهانی(1880) بوده است.

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۵، شماره ۶، صفحات ۱۱۴۰-۱۱۴۷

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023