rapid detection of escherichia coli o157: h7 by fluorescent amplification–based specific hybridization (flash) pcr
نویسندگان
چکیده
background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was successfully amplified in a flash–pcr. conclusion as rapid detection and confirmation of the presence of e. coli o157:h7 are of importance for the medical, food, and water industries, flash-pcr is one of selective methods for detection of e. coli o157:h7.
منابع مشابه
Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
BACKGROUND Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT PCR product of 336 bp was...
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عنوان ژورنال:
iranian red crescent medical journalجلد ۱۴، شماره ۹، صفحات ۵۹۴-۵۹۸
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