Nonspecific esterase zymograms of purified leukemic cells from a case of acute myelomonocytic leukemia (AMML) and from a case of acute non-B, non-T lymphocytic leukemia (ALL) were produced by isoelectric focusing and staining with alpha-naphthyl acetate (alpha NA) or alpha-naphthyl butyrate (alpha NB) substrate. A "myeloid" zymogram was found with AMML cells, which closely matched control monoc...

Protein phosphorylation is one of the most common post-translational modifications regulating many cellular processes. The phos-tag technology was combined with two-dimensional zymograms, which consisted of non-reducing IEF PAGE or NEPHGE in the first dimension and high resolution clear native electrophoresis (hrCNE) in the second dimension. The combination of these electrophoresis methods was ...

The formed elements of human blood each contain multiple isoenzymes of nonspecific esterase that hydrolyze short chain alpha naphthyl esters. Zymograms that are characteristic of each type of formed element are obtained by subjecting purified preparations of each to polyacrylamide slab gel electrophoresis at pH 9.5 and subsequent staining of the gels for esterase activity. The most prominent is...

the genus fusarium is a species rich genus. different media are required to study themorphological characters and classify different species, a time consuming technique. in this study pecticzymograms were used to identify species and form species of fusarium isolates. fusarium isolates (318) wereobtained from different areas and hosts in isfahan province, iran. isolates were identified to speci...

Electrophoresis is currently the best technique for quantifying inherited variation which has contributed to our knowledge of population biology in revealing population structure. Considerable variation in isozymes of diamondback moth, Plutella xylostella (L.), was detected. Esterase zymograms were prepared from 3rd and 4th instar larvae, pupae and adults. There were two groups of esterase band...

Honey is a sweet and healthy food produced by honeybees (Apis mellifera L.) from flower nectars. Using bidimensional zymography, we have detected the, until now unrevealed, proteolytic activities present in row honey samples. The resulting zymograms were specific for each type of the four unifloral honey under study, and enzymes were identified as serine proteases by the use of specific inhibit...