Background The sensitivity of smear microscopy for diagnosis of tuberculosis might be improved through treatment of sputum with sodium hypochlorite and application of fluorescent microscopy. This study aimed to determine the agreement between direct Fluorescent Microscopy and Ziehl-Neelsen concentration technique by their ability of detecting acid fast bacilli in resource poor settings. Metho...

AIMS To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS Best results were obtained by auramine O staining afte...

Background: Lymph node tuberculosis (LNTB) is the leading form of extra pulmonary tuberculosis and common in our country. Acid Fast Bacilli (AFB) detection by Ziehl-Neelsen (ZN) method in the fine needle aspirate from these lymph nodes is confirmatory. Objective: To assess the diagnostic utility of Ziehl-Neelsen staining in lymphadenopathy cases, since it is commonly used method in the diagnosi...

The presence of acid fast bacilli in sputum specimen was investigated comparatively with Ziehl-Neelsen (ZN)and Kinyoun Cold staining (KC) in order to determine sensitivity in detecting Tuberculosis Bacilli (T.B.). This study was employed on patients with severe lower respiratory tract infection to compare between these two stains. Which revealed that Ziehl-Neelsen procedure gives better results...

OBJECTIVE To evaluate the performance of GeneXpert for detection of mycobacterium tuberculosis in pus samples and compare its results with conventional techniques in terms of validity, rapidity and rifampicin resistance. METHODS This longitudinal, descriptive study was conducted at Jinnah Hospital, Lahore, Pakistan, from January 2012 to December 2015, and comprised pus samples of people suspe...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a new light-emitting, diode-based, fluorescence microscope, to compare auramine-rhodamine fluorescence with the conventional Ziehl-Ne...

Objective. To study the correlation of cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis with Ziehl-Neelsen staining (ZN), auramine-rhodamine staining (ARS), and autofluorescence (AF). Methods. A total of 145 lymph nodes were aspirated, 3 air-dried smears were stained with Giemsa, Ziehl-Neelsen, and auramine-rhodamine stains,...

microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. C...