The family of glycoprotein hormones and their homologous heptahelical receptors represent an excellent system for comparative structure-function studies. We have engineered single chain molecules of human chorionic gonadotropin (hCG) fused to its cognate receptor, LH receptor (LHR), and to the noncognate receptors, TSH receptor (TSHR) and FSH receptor (FSHR; N-beta-alpha-receptor-C), to create ...

Changes in the activities of antimicrobial, antitumor, and antioxidant properties postbiotics (YCG) are related to changes composition phenolic compounds. Antimicrobial activity was found be highest postbiotic (YCG-7) against P. aeruginosa, S. aureus, E. faecalis with an MIC 3.1 µg/mL. YCG-7 revealed most cytotoxicity LS-174T PC-3 cell lines IC50 5.78 6.56 µg/mL, respectively. far more effectiv...

In mammalian genomes CpGs occur at one-fifth their expected frequency. This is accepted as resulting from cytosine methylation and deamination of 5-methylcytosine leading to TpG and CpA dinucleotides. The corollary that a CpG deficit should correlate with TpG excess has not hitherto been systematically tested at a genomic level. I analyzed genome sequences (human, chimpanzee, mouse, pufferfish,...

N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated fusion enzymes that preferentially nick 5mCG-modified DNA. The CG dinucleotide can be modified b...

SARS coronavirus (SARS-CoV) papain-like protease (PLpro) has been identified in TGF-β1 up-regulation in human promonocytes (Proteomics 2012, 12: 3193-205). This study investigates the mechanisms of SARS-CoV PLpro-induced TGF-β1 promoter activation in human lung epithelial cells and mouse models. SARS-CoV PLpro dose- and time-dependently up-regulates TGF-β1 and vimentin in A549 cells. Dual lucif...

BACKGROUND Due to technological progress and improvements in medical care and health policy the average age of patients in primary care is continuously growing. In equal measure, an increasing proportion of mostly elderly primary care patients presents with multiple coexisting medical conditions. To properly assess the current situation of co- and multimorbidity, valid scientific data based on ...

We propose a new parameterization for describing forward and backward leanings of the straight human body with the feet fixed on the ground. During this motion stability of static equilibrium of the upright human body depends on the basic parameters such as coordinates XCG and YCG of the center of gravity of the body in the upright position and the radius R of the base of support. We introduce ...

YCG063 is known as an inhibitor of reactive oxygen species (ROS); however, its intracellular mechanisms of action remain poorly understood. In the present study, we investigated the effects of YCG063 on the inflammatory response of Pseudomonas aeruginosa lipopolysaccharide (PA-LPS)‑stimulated human retinal pigment epithelial cells (RPE cells). Human adult RPE cells (ARPE‑19) were stimulated wit...

TOPIC: Disorders of the Pleura TYPE: Medical Student/Resident Case Reports INTRODUCTION: Intrapleural fibrinolytic therapy is a common, surgery-avoiding option in treatment complicated para-pneumonic effusion. It has been shown to be safe majority patients, with most significant risk being severe pleural hemorrhage. In this case report, we will present unique patient who developed concurrent ma...