Xylella fastidiosa is a gram-negative, xylem-limited plant pathogenic bacterium that causes disease in a variety of economically important agricultural crops including Pierce's disease of grapevines. Xylella fastidiosa biofilms formed in the xylem vessels of plants play a key role in early colonization and pathogenicity by providing a protected niche and enhanced cell survival. Here we investig...

ABSTRACT Strains of Xylella fastidiosa, isolated from sweet orange trees (Citrus sinensis) and coffee trees (Coffea arabica) with symptoms of citrus variegated chlorosis and Requeima do Café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus PCR assays. These strains were also indis...

symptoms of the leaf scorch disease on the leaves of oleander infected by xylella fastidiosa appearing for the first time in lebanon were confirmed by elisa tests and anatomical observations of sections of petioles by a scanning electron microscope. xylella fastidiosa was detected in the xylem forming bacterial aggregates in the lumen of tracheary elements. the bacterium colonizes and invades t...

Citation: Cardinale M, Luvisi A, Meyer JB, Sabella E, De Bellis L, Cruz AC, Ampatzidis Y and Cherubini P (2018) Specific Fluorescence in Situ Hybridization (FISH) Test to Highlight Colonization of Xylem Vessels by Xylella fastidiosa in Naturally Infected Olive Trees (Olea europaea L.). Front. Plant Sci. 9:431. doi: 10.3389/fpls.2018.00431 Specific Fluorescence in Situ Hybridization (FISH) Test ...

Xylella fastidiosa, a pathogen of citrus, is the first plant pathogenic bacterium for which the complete genome sequence has been published. Inspection of the sequence reveals high relatedness to many genes of other pathogens, notably Xanthomonas campestris. Based on this, we suggest that Xylella possesses certain easily testable properties that contribute to pathogenicity. We also present some...

Phytoplasmas and Xylella spp. are bacteria that cause many economically important plant diseases worldwide. TaqMan probe-based quantitative real-time polymerase chain reaction (qPCR) assays have been utilized to universally detect phytoplasmas or Xylella fastidiosa. To develop a superior universal qPCR method, we used a dual priming oligonucleotide (DPO) with two annealing sites as a reverse pr...

Computational analyses of four bacterial genomes of the Xanthomonadaceae family reveal new unique genes that may be involved in adaptation, pathogenicity, and host specificity. The Xanthomonas genus presents 3636 unique genes distributed in 1470 families, while Xylella genus presents 1026 unique genes distributed in 375 families. Among Xanthomonas-specific genes, we highlight a large number of ...

Pierce’s disease (PD) causes symptoms of leaf scorch and fruit cluster wilt on wine, table and raisin grapes, and is caused by the bacterial pathogen Xylella fastidiosa. These fastidious, gram-negative bacteria occur only in the xylem of infected plants and are transmitted by xylem-feeding insects; leafhoppers and sharpshooters. The close association of plants with bacteria, either pathogenic o...

Following publication of our article [1], we found errors in analyses performed by the corresponding author (DJS) related to the phylogenetic relationship between Xylella species and the other xanthomonads. These errors do not make any difference to the main findings and conclusions reported in our paper. For example, the phylogenetic positions of NCPPB1131, NCPPB1132 and NCPPB4393 within the G...

The xylem limited plant pathogenic bacterium Xylella fastidiosa causes economically important diseases on agronomic, horticultural and landscape plants. This review includes the current status of polymerase chain reaction (PCR) based systems for detection and characterization of X. fastidiosa, and presents a genome-wide analysis of strain differentiation. The use of genomics data for strain com...