Histological Analysis and Immunohistochemistry Renal sections (3 μm thick) were stained with periodic acid-Schiff (PAS) and examined under light microscopy. Glomerulosclerosis index was semiquantitatively graded as 0 to 4+; vascular injury score as 0 to 3+; and tubulointerstitial injury score as 0 to 5+, according to the criteria reported previously. Ultrastructure of glomerular podocytes was a...

the intracellular parasite neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. for characterizeing the tachyzoite antigens of neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to neospora caninumin elisa. to ...

Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ antibody probing. To validate the microfluidic...

SKG or BALB/c T cells (3.0 £ 10) were stimulated for 72 h with plate-bound anti-CD3 mAb (2C11) in the presence or absence of plate-bound anti-CD28 mAb in RPMI-1640 medium supplemented with 10% fetal calf serum and 50 mM 2-mercaptoethanol. Cells were also stimulated with TPA (1.4 ng ml) and ionomycin (0.14 mM). KJ1.26þ T cells from DO or DO.SKG mice were stimulated with ovalbumin (323–339) pepti...