A pre-lysis buffer washing procedure was introduced to DNA extraction from a forest soil with high organic matter and iron oxide contents. Sodium phosphate of 0.1 M (pH 7.5) was used as a buffer to wash soil samples when subsequent lysis buffer was phosphate, and 20 mM EDTA (pH 7.5) was used when subsequent lysis buffer included EDTA. Initial experiments were not successful because the DNA extr...

We evaluated the effects of soil washing with ferric chloride (FeCl3) on cadmium (Cd) concentrations in soil solutions and Cd absorption by two spinach cultivars in pot experiments. Soil samples were collected from washed and unwashed plots of rice paddy fields in Fukuoka and Toyama, Japan. Spinach pot cultivation was performed with the above mentioned soil under oxidative conditions. Before an...

Introduction of fuel hydrocarbons and inorganic compounds (heavy metals) into the soil, resulting in a change of the soil quality, which is likely to affect use of the soil or endangering public health and ground water. This study aimed to determine a series of parameters to remediation of TPH and heavy metals contaminated soil by non-ionic surfactants- chelating agents washing process. In this...

Soil washing is one of the few permanent treatment alternatives to remove metal contaminants from soils. This paper reviews the various technology types and pilot/full-scale field applications of soil washing applicable to soils highly contaminated with heavy metals. The physical separation technologies, the chemical extraction processes and the integrated processes that combine both physical a...

The removal of cadmium and turbidity from contaminated soil-washing water was studied by Electroflotation (EF) at the laboratory scale by using sodium lauryl sulfate (SLS) as an anionic surfactant, and calcium hydroxide (Ca(OH)2) as a coagulant. The stoichiometric cadmium to collector (SLS) and coagulant (Ca(OH)2) ratio was 1:4.5. Significant removal of cadmium (100 %, not detected in the resid...

Citric acid (CA) was evaluated in terms of its efficiency as a biodegradable chelating agent, in removing zinc (Zn) from heavily contaminated soil, using a soil washing process. To determine preliminary ranges of variables in the washing process, single factor experiments were carried out with different CA concentrations, pH levels and washing times. Optimization of batch washing conditions fol...

the increasing production and use of fossil fuels increase the probability of soil source contamination by oil and petroleum products, and pose a hazard to life. there are over 43,000 chemical contaminated sites in canada and usa, with 2,200 of them classified as high risk. in the us, over $10 billion are spent annually on the cleanup of contaminated sites. the primary objective of this study w...

The combination of surfactant enhanced soil washing and degradation of nitrobenzene (NB) in effluent with persulfate was investigated to remediate NB contaminated soil. Aqueous solution of sodium dodecylbenzenesulfonate (SDBS, 24.0 mmol L-1) was used at a given mass ratio of solution to soil (20:1) to extract NB contaminated soil (47.3 mg kg-1), resulting in NB desorption removal efficient of 7...