We describe an investigation of the action of hyaluronidase on hyaluronan using viscosimetry. A new viscosimetric approach was developed for determining the activity of the enzyme in katal units. This approach requires knowledge of several parameters (e.g. Mark-Houwink constants) which were determined by combining viscosimetric measurement and gel-permeation chromatography analysis. Using all t...

The viscosimetric method of estimating enzyme concentration was first developed by Northrop and Hussey (1). In studies of the effect of irradiation of trypsin and pepsin solutions by Hussey and Thompson (2) certain modifications of the original methods were employed in order to decrease the opportunity for personal influence in curve plotting, as in extrapolation for the initial viscosity of th...

Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation changes in the region of aromatic residues near the sites responsible for acti...

The effect of partial autolysis during storage of shrimp biowaste on the quality of the extracted chitin and chitosan has been investigated. Shrimp waste materials were stored in a 30 o C incubator for 1-4 days. The quality of the chitin and chitosan extracted from these materials was assessed by ash and protein analysis and in solution by viscosimetry. Chitosan from fresh biowaste, treated for...

A viscosimetric method of estimating enzyme concentration with special reference to amylase has been discussed in another communication 1 from this laboratory; and, in a later report, * the effects of variation in the concentration of starch from the standard lot and variation in lot of prepared soluble starch have been contrasted, indicating that only within a small range of variation of p (de...