In HPLC, the detector is selected in accordance with the analyte. Many of the detectors used are based on optical absorbance, such as UV-Vis absorbance detectors and photodiode array (PDA) detectors. Absorbance detectors have the following features: The detector sensitivity and selectivity can be adjusted by setting an appropriate detector wavelength. The sensitivity is relatively high (althoug...

Wall-jet/thin-layer amperometric electrochemical detection (ECD) coupled with high-performance liquid chromatography (HPLC) was used here for the simultaneous analysis of dopamine (DA) and homovanillic acid (HVA) at a glassy carbon electrode. Compared with the conventional thin-layer mode and wall-jet mode, the presented wall-jet/thin-layer ECD has the advantages of enhanced capture of electroa...

Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250 mm×4.6 mm, 5 mm) using a mobile phase of 10 mM phosph...

A rapid, accurate, simple, and reproducible high performance liquid chromatographic (HPLC) method for the determination of oxytetracycline hydrochloride (OTC) in porcine plasma has been developed and validated. The drug and the standard were eluted from 5 μm Omni Spher (Varian) C18 column (250 mm × 4.6 mm I.D.) at room temperature. The mobile phase was composed of ACN-MeOH-(HCOO)2 (17.5:17.5:65...

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C(8) cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3....

A laboratory experiment was conducted by using three herbicides, two from dinitroaniline group and one from thiocarbamate group to know their degree of downward movement (leachability) through soil columns and their contribution in ground water contamination. Soil columns were loaded with Pendimethalin, Benthiocarb and Oryzalin at doses of 10.0, 10.0 and 7.7 kg/ha, respectively. After 30 days s...

Hyperlipidemia (HLP) is a group of lipid metabolism disorders with various pathogenesis. Its characteristic feature is an increase in cholesterol level, especially the low density lipoprotein fraction (LDL) level or triglyceride level in blood. An increase in total and LDL cholesterol level is related to the increased risk of ischemic heart disease as well as cerebral, coronary and peripheral c...

& In an HPLC system the detector is the component responsible for turning a physical or chemical attribute into a measureable signal corresponding to concentration or identity. In the early days of HPLC, detection was often carried out by collecting fractions and analyzing them off-line. It wasn’t until the 1940’s and 1950’s that the first online detectors for HPLC were introduced. Certainly an...