background and aims: virus-like particles (vlps) have been suggested to be a promising recombinant vaccine approach. several studies have reported that the influenza vlps produced in insect cells is an effective vaccine candidate. due to crucial role of matrix m1 protein in assembly and budding of influenza particles, in all vlps structures, m1 protein have been considered as a main component. ...

background preventing the seasonal or pandemic outbreak of influenza can be powerful and cost-effective. objectives in this study, we constructed a novel virus-like particle (vlp) platform that contains two hemagglutinin (ha) subtypes and evaluated immunogenicity of constructed vlp in mice. materials and methods this recombinant candidate vaccine model resulted in the expression of two has of h...

conclusions these findings of the present study indicate that the recombinant coree1e2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential hcv vaccine candidate. results in our study, the virus-like particle of rcoree1e2 with 70 nm size, was shown by electron microscopy and proved the self-assembly in vitro in a yeast expression system. materials and methods by ...

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background cucumber mosaic virus (cmv) has isometric particles with a diameter of about 28–29 nm. a useful method to recognize regions from mutagenesis in the coat protein (cp) gene are important for particle assembly and the expression of viral in cp expression systems like bacteria such as e.coli or yeast as well as their assembly into virus-like particles (vlp). objectives in this paper, we ...

This review presents data on commercial and experimental virus-like particle (VLP) vaccines, including description of VLP vaccines against influenza. Virus-like particles are multimeric, sometimes multiprotein nanostructures assembled from viral structural proteins and are devoid of any genetic material. VLPs present repetitive high-density displays of viral surface proteins. Importantly, they ...

Alphaviruses are comprised of a nucleocapsid core surrounded by a lipid membrane containing glycoprotein spikes. Previous work demonstrated that in vitro assembled core-like particles are similar in structure to the nucleocapsid core in the native virus. Here we demonstrate that in vitro assembled core-like particles can be inserted into viral glycoprotein-expressing cells to generate enveloped...