نتایج جستجو برای: virus growth
تعداد نتایج: 1198353 فیلتر نتایج به سال:
knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old ece...
Knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. The present study aimed to evaluate the best harvesting time of H9N2 avian influenza virus (AIV) vaccine strain inoculated in specific pathogen free (SPF) embryonated chicken eggs (ECE)s. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated into 336, 11-day old ECE...
Introduction: Avian influenza viruses are considered as a serious threat to human and animal health. An increase in expression of proinflammatory cytokines and type I IFN genes, as well as host cell death responses contribute to the pathogenesis of influenza infection. Hence, this study aimed to evaluate the growth dynamics of subacute avian influenza virus in human respiratory alveolar epithel...
African yam bean (Sphenostylis stenocarpa) is an underexploited legume with the potential of becoming an alternative food staple to alleviate the problem of food security. An assessment was carried out on the growth and yield of AYB affected by Telfairia mosaic virus (TeMV) Infection. Telfairia mosaic virus caused severe mosaic, leaf distortion and malformation on AYB. Analyses of results revea...
Background and Objectives: In this study, capability of Vero cells for growth on FibraCel disks were compared on 3 kinds of microcarriers including Cytodex-1, Cutodex-3, and Sigma Solohill in 500 ml Spinner flasks in both serum contained medium (DMEM+10% Fetal Calf Serum, FCS) and serum-free medium (VP-SFM). The propagation of fixed PV (Pasteur Virus) strain of rabies in Vero cells, for product...
In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media. Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishik...
In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media. Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishik...
Duck beak atrophy and dwarfism syndrome (BADS) is a newly emerged disease in China since 2015. In October 2017, an unidentified disease occurred in Cherry Valley ducks, Chongqing municipality, the southwest of China. The affected birds showed short beak and growth retardation clinical signs. The disease caused approximately 20.00% morbidity and serious weight loss due to retarded growth. In ord...
determining influenza virus shedding at different time points in madin-darby canine kidney cell line
objective: monitoring of influenza virus shedding and optimization of multiplicities of infection (moi) is important in the investigation of a virus one step growth cycle and for obtaining a high yield of virus in vaccine development and conventional basic diagnostic methods. however, eluted infectious viruses may still be present immediately after virus inoculation and when cells are washed fo...
Background and Aims: Inhibition of viral growth in coinfected cells with two different viruses has been described. This phenomenon known as viral interference can occur in several virus host systems such as interference of enterovirus infection on poliovirus vaccine strains. In this study we superinfected reovirus infected HeLa cells with poliovirus to determine if poliovirus can replicate in s...
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