this research was performed in order to study and diagnose potato virus y,potyvirus (pvy), potato virus x,potexvirus (pvx), potato virus s,carlavirus (pvs), potato virus a,potyvirus(pva) and potato leafroll luteovirus (plrv) on tubers and leaves of different potato varieties, namely, solea, safran, floris, proventa, milva, universa, lady olympia, vangogh, and marabel grown in afyon region of tu...

background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

marek’s disease (md) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. the present study was an attempt to use polymerase chain reaction (pcr) tooptimize a rapid and reliable assay for detection of mdv genome. detection of serotype 1 of mdv (mdv-1)was confirmed by presence of a 200 bp dna fragment as a pcr product. differentiation of mdv-1 ...

Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...

infectious bronchitis virus (ibv) belongs to the group of gamma coronaviruses along with other avian coronaviruses. the disease caused by ibv can appear similar to infectious laryngotracheitis, avian influenza, and velogenic newcastle disease, which are high priority diseases. the clinical signs can be accompanied by mortalities in broiler chickens and reduced eggshell and albumin quality in la...

Avian influenza H9N2 subtype viruses have had a great impact on Iranian industrial poultry production economy since introduction in the country. To approach Rapid and precise identification of this viruses as control measures in poultry industry, a real time probe base assay was developed to directly detect a specific influenza virus of H9N2 subtype -instead of general detection of Influenza A ...

background and aims: considering difficulties in usual laboratory methods in detection of viral infections, improved dna-based diagnostic techniques are more reliable. loop mediated isothermal amplification method (lamp) is a nucleic acid amplification method that amplifies dna using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. in this study, the...

Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans. Highly pathogenic avian influenza viruses (HPAI) H5N1 were first identified in Southeast Asia in 1996 and spread to four continents over the following years. The viruses have caused high mortality in chickens and various bird species ...

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...

BK virus-associated hemorrhagic cystitis (HC) is frequent in patients undergoing bone marrow transplantation, but it rarely occurs among other immunosuppressed patients particularly in those who are receiving chemotherapy for hematologic malignancies. Herein, we report three children with malignancies suffering from hemorrhagic cystitis due to BK virus infection who were successfully improved. ...