quick identification of vibrio cholerae in epidemics is   important, on the other hand; conventional methods are time-consuming and   costly. the aim of this study was to develop a rapid, inexpensive and high   sensitivity method for quick identification of vibrio cholerae . for   this purpose we designed a pcr detection based on magnetic nanoparticles for   identification of bacterial dna by p...

mono-specific antisera against vibrio cholera ogawa nih-43 and vibrio cholera inaba 35-a3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. to visualize antigen-antibody reaction, gamma globulins were conjugated to staphylococcus aureus cowan-l (nctc: 8325) in th...

background several hundred thousand people are infected with vibrio cholera every year worldwide, with a case fatality rate over 2%. periodic cholera outbreaks are of important health issues in iran, especially in sistan and baluchistan province. objectives in this study, we evaluated cholera out breaks and related factors, during 2010-2013, to improve controlling the disease in this province. ...

Introduction.?Koch in 1883 isolated the cholera vibrio from cholera patients in Calcutta. Tinkler and Prior (1884) and Deneke (1885) described vibrios resembling Koch's vibrio that had been isolated from non-choleraic material. In the twelve years following the discovery of the cholera vibrio, Metchnikof, Sanarelli, Gunter, Foker oftid other workers isolated vibrios indistinguishable morphologi...

materials and methods from 2004 to 2005, plants were collected from various ecological niches and the roots were used to isolate v. cholera. standard bacteriological, biochemical and serological tests were used for its typification. results in five out of the nine ecological niches explored, we collected either l. minor or e. crassipens, as these specimens cohabited only in two niches. v. chole...

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       In this investigation, a...

Background: Cholera disease caused by Vibrio cholerae remains a major cause of morbidity and mortality throughout the world. Various strategies with different proteins as immunogens have been tried for vaccine development, none of which have been sufficiently effective to preclude cholera. Chimeric proteins, with their ability to present multiple antigens at the same time, can play important ro...

The origin of cholera has been elusive, even though scientific evidence clearly shows it is a waterborne disease. However, standard bacteriological procedures for isolation of the cholera vibrio from environmental samples, including water, between epidemics generally were unsuccessful. Vibrio cholerae, a marine vibrio, requiring salt for growth, enters into a dormant, viable but nonculturable s...