Verticillium wilt is a major threat to alfalfa (Medicago sativa) and many other crops. The model legume Medicago truncatula was used as a host for studying resistance and susceptibility to Verticillium albo-atrum. In addition to presenting well-established genetic resources, this wild plant species enables to investigate biodiversity of the response to the pathogen and putative crosstalk betwee...

A fractions obtained from the culture fluids of a seven fungal species identified as Verticillium albo-atrum; V. lecanii; V. bulbillosum; V. dahliae; Fusarium solani; F. oxysporum, and Aspergillus sclerociarum were shown to contain a compounds with a biological activity against twenty two clinical bacterial strains including eleven isolates of gram positive and other were gram negative. Among t...

Accurate species identification is essential for effective plant disease management, but is challenging in fungi including Verticillium sensu stricto (Ascomycota, Sordariomycetes, Plectosphaerellaceae), a small genus of ten species that includes important plant pathogens. Here we present fifteen PCR assays for the identification of all recognized Verticillium species and the three lineages of t...

The vascular wilt fungi Verticillium dahliae and V. albo-atrum infect over 200 plant species, causing billions of dollars in annual crop losses. The characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels, which undergo fluctuations in osmolarity. To gain insights into the mechanisms that confer the organisms' pathogenicity and enable th...

ABSTRACT A precise real-time polymerase chain reaction (PCR) assay was developed for quantifying Verticillium albo-atrum DNA. The assay was used in a repeated experiment to examine the relationship between the quantity of pathogen DNA detected in infected leaves and shoots and the severity of Verticillium wilt symptoms in several alfalfa cultivars expressing a range of disease symptoms. Plants ...

Molecular approaches were used to re-evaluate the morphological criteria used to identify plant-associated Verticillium species. ITS-RFLPs divided the 31 studied strains of seven Verticillium species, (including the type species V. luteo-album) into four clusters. Cluster one comprised strains of the type species, V. luteo-album, cluster two V. albo-atrum, V. dahliae, V. nubilum and V. tricorpu...

There are few reports of the application of antibiosis as a possible means of controlling Verticillium alboatrum Reinke and Berthold. Most of these publications are concerned with in vitro studies which report the effects of various organisms or their products on V. albo-atrum (Ark and Hunt, 1941; Arnstein et al., 1948; Smith, 1947). Wilhelm (1951a) effected a reduction in the inoculum potentia...

Knowledge of pathogen biology and genetic diversity is a cornerstone of effective disease management, and accurate identification of the pathogen is a foundation of pathogen biology. Species names provide an ideal framework for storage and retrieval of relevant information, a system that is contingent on a clear understanding of species boundaries and consistent species identification. Verticil...

Enzymatic activity e.g., pectinase, cellulase, protease and lipase of different fungal pathogens causing stalk rot disease in corn was determined. In-vitro studies; corn stalk rot pathogens viz., Cephalosporium acremonium, Fusarium moniliforme, F. graminearum, F. semitectum, Macrophomina phaseolina, st. 1, M. phaseolina st. 2, Rhizoctonia solani st.1, R. solani st. 2 and Verticillium albo-atrum...

The influence of Pratylenchus penetrans on the incidence and severity of Verticillium wilt was examined in the potato cultivars 'Kennebec', 'Katahdin', and 'Abnaki'. Single-stem plants were grown in soil maintained at a temperature of 22 +/- 1 C. Axenically cultured nematodes were suspended in water and introduced to the soil, at a rate of ca 5,000/25.4-cm pot, through holes made around each st...