Nontreponemal antibody tests such as the Venereal Disease Research Laboratory (VDRL) test are carried out on serum and widely used as screening tests for syphilis. The aim of the present study was to develop a screening test for syphilis making use of whole blood and VDRL liposomes. Antibody to human red blood cells was conjugated to VDRL liposomes and reacted with a diluted sample of patient w...

OBJECTIVE In this study, we aimed to determine the performance characteristics of toluidine red unheated serum test on cerebrospinal fluids (CSF-TRUST) as compared to venereal disease research laboratory test on cerebrospinal fluids (CSF-VDRL) for laboratory the diagnosis of neurosyphilis. DESIGN A cross-sectional study. SETTING Sexually transmitted infections (STIs) clinics. PARTICIPANTS...

An enzyme-linked immunosorbent assay (ELISA) for detection of immunoglobulin G (IgG) and IgM to cardiolipin, lecithin, and cholesterol (VDRL [Venereal Disease Research Laboratory] ELISA) is described. The specificity of the VDRL ELISA for IgG and IgM was 99.6 and 99.5%, respectively, with sera from 1,008 persons without syphilis. For a group of patients with false-positive results in traditiona...

Background. More than 1 million newborns are infected yearly worldwide making from congenital syphilis a global concern. In 2009 in Romania were reported rates of global seroprevalence of 15 per 100.000 population representing the highest rate in Europe. Study design. A retrospective observational study was carried out, starting from January 2011 to December 2012, on syphilis infection in pregn...

OBJECTIVES We evaluated the use of treponemal serum tests in cerebrospinal fluid (CSF) to diagnose neurosyphilis since CSF-Venereal Disease Research Laboratory (VDRL) is specific but lacks sensitivity. METHODS We tested CSF specimens using the following treponemal serum tests: INNO-LIA, Treponema pallidum particle agglutination (TP-PA), Trep-Sure, and Maxi-Syph. The reference standard to calc...

In this study, we aimed to investigate the performance of nontreponemal antibody tests in cerebrospinal fluid (CSF) specimens from syphilis patients. From September 2009 to September 2012, CSF specimens were collected at the Shanghai Skin Disease Hospital in Shanghai, China, from 1,132 syphilis patients without HIV infection, including 154 with symptomatic and 56 with asymptomatic neurosyphilis...

Of 9733 consecutive serological samples received by Portsmouth and Southampton Public Health Laboratories (PHL) and tested for syphilis, 190 (140 from men and 50 from women) gave positive results. Thirty new cases of syphilis were identified. Most sera were tested initially by both a specific antibody test (the Treponema pallidum haemaglutination (TPHA) test) and a cardiolipin test (the Venerea...

Results These are presented in table 1. Owing to the unavailability of the Treponema pallidum haemagglutination assay (TPHA) reagents at certain times and the inability of some women to aVord the cost of screening, only 30 of the 148 VDRL reactive serum samples were subjected to TPHA, with three being positive. Thus, 10% of the VDRL reactive sera were also TPHA reactive. This TPHA/VDRL ratio is...

The rapid plasma reagin (RPR) card test manufactured by Beckman Instruments, Inc., was compared, qualitatively and quantitatively, with the Venereal Disease Research Laboratory (VDRL) slide test and the standard RPR 18-mm circle card tests for the serodiagnosis of syphilis. Sera from 638 individuals were used in this study. Two pilot lots and two production lots of antigen were submitted by Bec...

Presently, the screening of syphilis is dependent mainly on serological tests. In the sexually transmitted disease clinic, syphilis serology is a basic screening test. The results VDRL test among of 150 HIV (82 males, 68 females) infected, regardless to immune status (CD4+ count), were studied. It was found that in 2 cases, the VDRL was biologically false reactive (VDRL positive, TPHA negative)...