conclusions full factorial design experimental approach helped to identify the critical culture condition parameters in heterologous expression of v2r. it would allow studying the parameters affecting the function of this protein in future experiments. results the maximum amount of protein production was obtained by the addition of 0.75 mm iptg at 37°c after three hours. the most important fact...

the vasopressin v2 receptor belongs to the large family of the g-protein coupled receptors and is responsible for the antidiuretic effect of the neurohypophyseal hormone arginine vasopressin (avp). based on bioinformatic studies it seems that ala300 and asp297 of the v2 vasopressin receptor (v2r) are involved in receptor binding. ala300glu mutation resulted in lower energy while asp297tyr mutat...

Hyponatremia (hypo-osmolality) is a disorder of water homeostasis due to abnormal renal diluting capacity. The body limits the degree which serum sodium concentration falls through mechanism called “vasopressin escape”. Vasopressin escape process that prevents continuous decrease in even under conditions sustained high plasma vasopressin levels. Previous reports suggest aldosterone may be invol...

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...

Nociceptin, the endogenous ligand of the inhibitory G protein-coupled opioid receptor-like 1 receptor, produces aquaresis (i.e., increases the excretion of solute-free urine) in rats. However, the mechanism underlying this effect has not yet been explained. Using immunohistochemistry, we found the opioid receptor-like 1 receptor in the rat kidney colocalized with the vasopressin-regulated water...

the antidiuretic effect of arginine vasopressin (avp) is mediated by the vasopressin v2 receptor. the docking study of avp as a ligand to v2 receptor helps in identifying important amino acid residues that might be involved in avp binding for predicting the lowest free energy state of the protein complex. whereas previous researchers were not able to detect the exact site of the ligand-receptor...

septic shock continues to be one of the leading causes of death in the intensive care units. when the shock state persists after adequate fluid resuscitation,  vasopressor therapy is required to improve and maintain adequate tissue/organ  perfusion in an attempt to improve survival and prevent the development of multiple organ dysfunction and failure. various studies have suggested that exogeno...

Heritable nephrogenic diabetes insipidus (NDI) is characterized by defective urine concentration mechanisms in the kidney, which are mainly caused by loss-of-function mutations in the vasopressin type 2 receptor. For the treatment of heritable NDI, novel strategies that bypass the defective vasopressin type 2 receptor are required to activate the aquaporin-2 (AQP2) water channel. Here we show t...