Tanaman anggrek merupakan tanaman hias yang memiliki keanekaragaman tinggi dan tersebar luas di Indonesia. diperkirakan sekitar 5000 spesies hutan-hutan Salah satu asli Indonesia adalah Coelogyne dayana Rchb.f. Tujuan penelitian ini untuk mengetahui media tanam paling baik C. dayana. Penelitian menggunakan metode deskriptif membandingkan perlakuan 3 jenis berbeda yaitu T1V (Vacin & Went per...

The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem's Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusA gene expres...

To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm diameter) Sw. derived from MS supplemented with 0.5 mg/L TDZ 60 days culture were cultured on 6 media; Murashige Skoog (MS) medium, 15 % (v/v) CW, CW 0.2 (w/v) AC, Vacin Went (VW) VW AC. After months culture, containing coco...

Phalaenopsis venosa Shim & Fowlie adalah anggrek endemik Sulawesi terancam punah sehingga perlu dilakukan upaya konservasi. Tujuan penelitian untuk mengetahui mikromorfologi dan media perkecambahan in vitro mendukung konservasi P. venosa. Biji dipanen pada enam bulan setelah penyerbukan disimpan di freezer suhu -4 °C selama sembilan bulan. Pengamatan menggunakan mikroskop cahaya OPTIKA M-69...

Dendrobium is a genus of orchids that has been widely planted, both domestically and internationally. Orchid conventional breeding generally done to produce new variants, but seeds from crosses are difficult germinate. To solve this problem, tissue culture long used germinate orchid seeds. This study aims obtain suitable medium composition for growing 'Bertachong' X 'Blackspider x Sutiknoi'. Th...

As interest in how to increase biomass production through biotechnological means gains traction, focus is turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevated CO2 with cold cathode fluorescent lamps (CCFLs) on the photoautotrophic growth of Oncidesa in vitro has been stu...

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...

Abstract. Setiaji A, Annisa RRR, Santoso AD, Kinasih Riyadi ADR. 2021. Review: Factors affecting mass propagation of Vanda orchid in vitro. Cell Biol Dev 5: 51-62. For the past decade, has been primadonna ornamental orchids south and southeast Asia, along with Phalaenopsis Dendrobium. Along increase demand for Vanda, this genus faced several threats, from illegal collection to habitat loss. The...