introduction: vaccinology provides promising approaches for the control of various infectious diseases. among different strategies, dna vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. however, there is a need to increase their potency by the us...

background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: struct...

background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...

background and aims: influenza (flu) is a respiratory infection in mammals and birds. it is caused by an rna virus in the family orthomyxoviridae. the virus is divided into three main types. influenza virus type a is found in a wide variety of bird and mammal species and can undergo major shifts in immunological properties. hemagglutinin (ha) is an important influenza virus surface antigen that...

background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Background: Hepatitis C (HCV) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. DNA vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. Objectives: In the present study, a DNA vaccine expressing HCV core protein was developed with IL12 as a genetic a...

background: dna vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. bacteria of the genus brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. methods: to evaluate the use of a dna immunization strategy for protection against brucellosis, a plasmi...

toxoplasmosis is an infection caused by the protozoan parasite toxoplasma gondii (t.gondii) throughout the world. although usually asymptomatic, the infection can cause serious medical problems in immunocompromised individuals and fetuses. toxoplasmosis also causes considerable economic loss because of abortion in livestock. dna vaccination is a promising approach against intracellular parasite...