conclusions polymerase chain reaction has specificity and accuracy for identification of the organism and is able to differentiate biotypes. enterobacterial repetitive intergenic consensus sequence is one of the informative and discriminative methods for the analysis of v. cholerae diversity. the rep-pcr is a less informative and discriminative method compared to other methods for the analysis ...

conclusions obtained data demonstrated that the designed assay is an accurate, easy, rapid, and cost-effective tool for detecting v. cholerae that can serve as an alternate to current bacteriological tests. background several epidemic and endemic cases have been reported involving vibrio cholerae (v. cholerae) as a causative organism in serious diarrheal diseases with high mortality. hence, qui...

cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time...

Vibrio cholerae O139 is a recently identified non-O1 V. cholerae strain responsible for outbreaks of epidemic cholera in India, Bangladesh, and Thailand in the past 2 years. Other workers have demonstrated the presence of the cholera toxin genetic element in V. cholerae O139, unlike the situation for other non-O1 V. cholerae strains. We sought to compare further this strain with strains of V. c...

vibrio cholerae o1 are classified into two biotypes, classical and el tor based on susceptibility to bacteriophages and some biochemical properties, each encoding a biotype-specific genetic determinants. before 1961, most epidemics had been caused by the classical biotype. however, with the passage of time, the classical biotype missed from the scenario and the el tor emerged as the major bioty...

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...

At the International Centre for Diarrhoeal Disease Research, Bangladesh, one-half of the rice-water stool samples that were culture-positive for Vibrio cholerae did not contain motile V. cholerae by standard darkfield microscopy and were defined as darkfield-negative (DF(-)). We evaluated the host and microbial factors associated with DF status, as well as the impact of DF status on transmissio...

The bacterium Vibrio cholerae, the etiological agent of cholera, is often found attached to plankton, a property that is thought to contribute to its environmental persistence in aquatic habitats. The V. cholerae O1 El Tor biotype and V. cholerae O139 strains produce a surface pilus termed the mannose-sensitive hemagglutinin (MSHA), whereas V. cholerae O1 classical biotype strains do not. Altho...

The National Institute of Communicable Diseases (NICD), Delhi, India, conducts a laboratory-based surveillance of cholera cases referred from the Infectious Disease Hospital, Delhi. The prevalence and antimicrobial susceptibilities of Vibrio cholerae O1, O139, and others, isolated from cholera patients for nine years, were analyzed to determine the changing trends in their isolation and drug-re...

Title of Dissertation / Thesis: INTERACTIONS OF VIBRIO CHOLERAE SEROGROUPS O1 AND O139 AND COPEPODS Tonya Kafí Rawlings, Doctor of Philosophy, 2005 Dissertation / Thesis Directed By: Dr. Rita R. Colwell, Department of Cell Biology and Molecular Genetics Vibrio cholerae O1 El Tor and O139 Bengal have caused cholera epidemics throughout Bangladesh and elsewhere in the world with a seasonal regula...