Dynein axonemal light intermediate chain 1 (dnali1) is an important part of dyneins and plays role in the growth development animals. However, there little information about dnali1 fish. Herein, we cloned gene from genome olive flounder (Paralichthys olivaceus), a commercially maricultured fish China, Japan, Korea, analyzed its expression patterns different gender The DNA sequence contained 771...

Objective(s):OCT4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. Therefore, the effective expression regulation of this gene is highly critical. UTR regions are of great significance to gene regulation. In this study, we aimed to investigate the potential regulatory role played by 5´UT...

Abstract The neurotrophin Brain-derived neurotrophic factor (BDNF) is encoded by multiple bipartite transcripts. Each BDNF transcript composed one out of 11 alternatively spliced exons containing the 5′untranslated region (UTR), and common exon encompassing coding sequence (CDS) 3′UTR with two variants (short long). In neurons, mRNA have a distinct subcellular distribution, constituting “spatia...

Silencing XB130 inhibits cell proliferation and epithelial‑mesenchymal transition in non‑small lung cancer (NSCLC), suggesting that downregulating expression may impede NSCLC progression. However, the molecular mechanism underlying regulation of remains unclear. In present study, role 3'‑untranslated region (3'‑UTR) was investigated. Recombinant psiCHECK‑2 vectors with wild‑type, truncated, or ...

objective(s):oct4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. therefore, the effective expression regulation of this gene is highly critical. utr regions are of great significance to gene regulation. in this study, we aimed to investigate the potential regulatory role played by 5´ut...

Rapid detection and differentiation of infectious bronchitis virus (IBV) involved in the disease outbreak is very important for controlling disease and developing new vaccines. In the present study, three regions of the genome of IBV vaccine and field isolates including S1 gene, gene 3 and nucleocapsid (N) gene along with 3' untranslated region (3' UTR) were amplified and subjected to restricti...

The present study was conducted on the Nile tilapia (Oreochromis niloticus) and screened for single nucleotide polymorphisms (SNPs) in muscle hormone genes myogenin (MyoG) myostatin (MSTN). Samples were collected from JangHang fish farm categorized by body weight sex. average total 1.02 kg 82 males 0.63 70 females. A range of ±10% considered as normal weight. Target regions each gene categorize...