نتایج جستجو برای: using chinese spring genomic dna as probe

تعداد نتایج: 7839016  

Journal: :پژوهش های تولید گیاهی 0

to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...

ژورنال: علوم آب و خاک 2009
باقی زاده, امین, خنامانی فلاحتی پور, سوده, شاهسوند حسنی , حسین, کریم زاده, قاسم,

The genomic in situ hybridization (GISH) has been used to identify euploidy and aneuploidy in segregation generations of various plants. In this study, the GISH with minor modifications including, slide preparation of putative secondary Tritipyrum (F2) root meristemic cells, labeled genomic DNA of Thinopyrum bessarabicum by fluorescein 12-dUTP nucleotide as probe, genomic DNA of Thinopyrum bess...

باقی زاده, امین, خنامانی فلاحتی پور, سوده, شاهسوند حسنی , حسین, کریم زاده, قاسم,

The genomic in situ hybridization (GISH) has been used to identify euploidy and aneuploidy in segregation generations of various plants. In this study, the GISH with minor modifications including, slide preparation of putative secondary Tritipyrum (F2) root meristemic cells, labeled genomic DNA of Thinopyrum bessarabicum by fluorescein 12-dUTP nucleotide as probe, genomic DNA of Thinopyrum bess...

نزهت, زهرا , هدایتی, مهدی ,

In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...

The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...

Journal: :iranian journal of veterinary research 2010
a. jolodar

aspartic proteases are a relatively small group of enzymes which express in various nematodes including onchocerca volvulus. an estimation of the gene copy number corresponding to the ov7a clone, which contains a cdna insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of o. volvulus, was made by slot blot analysis in a closely related species o. gibsonig...

Journal: :avicenna journal of medical biotechnology 0

genomic signal processing is a relatively new field in bioinformatics, in which signal processing algorithms and methods are used to study functional structures in the dna. an appropriate mapping of the dna sequence into one or more numerical sequences enables the use of many digital signal processing tools in the analysis of different genomic sequences. also, a novel influenza a (h1n1) virus o...

Journal: :caspian journal of environmental sciences 2011
s. a. shirangi

the main objective of this study was to assess genetic comparison of two migratory forms of caspian trout salmo trutta caspius namely fall-run and spring-run. owing to the lack of information on its genetic differences, 5 microsatellite loci were used for 58 sample analyses. genomic dna was extracted from caudal fin using roche dna extraction kit and each pcr reaction was performed in a 25 ?l r...

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