نتایج جستجو برای: universal pcr
تعداد نتایج: 279379 فیلتر نتایج به سال:
background: the clinical diagnosis of meningitis is crucial, particularly in children. the early diagnosis and empiric antibiotic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16srdna fragment which is produced by universal primers led up fast and sensitive determination. the purpose of this study was to investigate a rapid method fo...
background and objectives: urinary tract infection (uti) is one of the most common infections and a major cause of patient morbidity world wide. the gold standard for detection of bacterial pathogens in uti is culture of urine specimens. in order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in uti. ma...
سابقه و هدف: کاوازاکی بیماری مهم و خطرناک با عوارض وخیم اما قابل پیشگیری می باشد، به شرطی که زود تشخیص داده شود و درمان گردد. علی رغم سال ها تلاش و تحقیق، هنوز عوامل ایجاد بیماری کاوازاکی ناشناخته باقی مانده است.مواد و روش ها: کلیه بیماران مبتلا به سندرم کاوازاکی که در مرحله حاد در سال های 1386-1385 به مرکز طبی کودکان مراجعه نمودند و قبلا ivig (گاما گلوبولین وریدی) نگرفتند در مطالعه قرار گرفتند...
Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
background: there is a conserved portion in the 16s rrna gene of bacteria which can be amplified by the universal pcr method. this fragment is 996 bp in length. in this method, only one set of universal primers is used for the amplification of the conserved region of the 16s rrna gene, in common bacterial pathogens. therefore, using the universal pcr method, these bacteria are detectable only b...
INTRODUCTION Central nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patients who were referred to Koodakan Hospital in Bandar Abbas because they were suspected of having th...
BACKGROUND Specific and sensitive nucleic acid (NA) testing in research and clinical diagnostics is usually performed by use of labeled oligonucleotide probes. However, the use of target-specific fluorogenic probes increases the cost of analysis. Therefore, universal sequence-dependent (USD) NA detection methods have been developed to facilitate cost-effective target detection using standardize...
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