Acinetobacter baumannii is an opportunistic pathogen that is frequently involved in outbreaks of infection, occurring mostly in intensive care units. The increasing incidence of carbapenem resistance in A. baumannii worldwide is a concern since it limits drastically the range of therapeutic alternatives. The most important mechanism of carbapenem resistance is the enzymatic hydrolysis mediated ...

background and objectives: klebsiella pneumoniae is an opportunistic pathogen responsible for up to 10% of nosocomial infections. the emergence and spread of multidrug resistant k. pneumoniae , mostly due to the production of extended-spec- trum β-lactamases (esbl) and carbapenemases, is often responsible for antibiotic treatment failure of these infections. we compared the antibiotic resistanc...

The rapid spread of gram-negative bacteria resistance to carbapenems due the production carbapenemases requires new treatment options. activity carbapenem antibiotic biapenem, recently registered in Russia, against producers various was studied comparison with other antibiotics this group. Among NDM-type carbapenemase producers, 77.8% demonstrated clinical susceptibility biapenem; 50.3% and 21....

The most important mechanism of carbapenem resistance in Enterobacteriaceae is the production of carbapenemases, although resistance can also result from the synergistic activity between AmpC-type or (to a lesser extent) extended-spectrum beta-lactamases combined with decreased outer membrane permeability. Three major molecular classes of carbapenemases are recognized: A, B and D. Classes A and...

The emergence of carbapenem-resistant Gram-negative pathogens poses a serious threat to public health worldwide. In particular, the increasing prevalence of carbapenem-resistant Klebsiella pneumoniae is a major source of concern. K. pneumoniae carbapenemases (KPCs) and carbapenemases of the oxacillinase-48 (OXA-48) type have been reported worldwide. New Delhi metallo-β-lactamase (NDM) carbapene...

The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing carbapenemase. Carbapenemase production was verified by PCR and UV-spectrophotometric measurement o...

background hospital-acquired infections caused by multi-drug resistant acinetobacter spp. are often extremely difficult to treatand this has proved to be a serious problem worldwide. objectives the aim of this study was to determine the incidence rates and distribution patterns of multi-drug resistant (mdr) acinetobacter spp. strains and the occurrence of acinetobacter-derived cephalosporinase ...

Carbapenem-resistant Enterobacteriaceae are increasingly reported worldwide and cause therapeutic problem in health care facilities. In this study 28 imipenem-resistant K. pneumoniae were examined for expression of carbapenemases by phenotypic and genotypic methods. Modified Hodge Test (MHT), CarbaNP test were used for phenotypic detection, and PCR using specific primers for the detection of bl...

Nowadays, the infections due to carbapenem-resistant microorganism represent a global public health issue; microorganisms most frequently involved are gram-negative bacteria. This pose major threat because of their elevated mortality, lack appropiate antibiotics and its rapid spread arround world. The emergence carbapenemases, which type enzimes that hydrolize carbapenems, is resistance mecanis...