objective: polymerase chain reaction (pcr) is one of the most important progress in the field of molecular biology and diagnosis. despite simplicity in concept, the reaction needs complex interaction between target sequence, primers, dntps and dna polymerase for a successful amplification and diagnosis. for the detection of rna viruses highly sensetive and specific technique is required. hence ...

tomato is an important vegetable crop in iran. recently symptoms associated with phytoplasma disease were observed in tomato cultivars in karaj vicinity. the phytoplasmas were detected and characterized using single-tube nested pcr and rflp. the universal primer pairs amplified the target dna with expected sizes from symptomatic samples in direct pcr and single-tube nested pcr. the patterns of ...

A novel method, which involves a nested PCR in a single closed tube, was developed for the sensitive detection of Erwinia amylovora in plant material. The external and internal primer pairs used had different annealing temperatures and directed the amplification of a specific DNA fragment from plasmid pEA29. The procedure involved two consecutive PCRs, the first of which was performed at a high...

Check out our Selection Guide! PCR RNA DNA LAMP KitsOne Tube For Superior Reproducibility. From fish tissues or fluids using a nested PCR primer set. The use of trade, firm, or corporation names in this protocol is for the information and.jor consideration in designing a nested PCR protocol compatible with quantitation is to assure that the maximum concentration of PCR products produced by the....

Toward the goal of reducing diagnostic false positives while retaining high sensitivity, a closed-tube nested PCR procedure has been developed for detecting low-copy-number human immunodeficiency virus (HIV) gag target DNA sequences. Master mix for amplification 2, in a hanging gel matrix at the reaction tube top, remains sequestered from the reaction space of the tube during amplification 1. A...

An ultra-sensitive and quantitative diagnostic system by combining nested PCR and TaqMan PCR in a single tube was developed for detection of "Candidatus Liberibacter asiaticus". The procedure involves two PCR steps using the species-specific outer and inner primer pairs. Different annealing temperatures allow both the first and the second rounds of PCR to be performed sequentially in the same c...

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...

Institut f~r Laboratoriumsund Transfusionsmedizin, Herzund Diabeteszentrum Nordrhein-Westfalen, Universit~itsklinik der Ruhr-Universit~it Bochum, Bad Oeynhausen, Germany The application of nested PCR in medical diagnostics implies that the same type of reaction is performed repetitively in the same laboratory over long periods of time, such as in the longitudinal monitoring of immunosuppressed ...