The goal of the present study was to compare the nuclear phosphoproteins of normal and thioacetamide-treated rat liver, which are nondividing tissues, with regenerating rat liver and Novikoff hepatoma, which are growing and divid ing tissues. Thioacetamide treatment was utilized to induce nucleolar hypertrophy (1, 9). With the increased resolution of the 2-dimensional elec trophoresis method, (...

In March 2009, six cases of invasive meningococ-cal disease (IMD) were reported in two communes of Goleniów County in northwest Poland. The people affected were aged from seven to 25 years. The overall incidence rate in the county was eight per 100,000 population. All the patients recovered fully. No epidemiological links were established between the cases. All were infected with Neisseria meni...

background: the effects of extremely low frequency electromagnetic fields (elf-emf) on toxoplasma gondii have not been explained yet. the aim of this study was to assess the possible effects of elf-emf on growth, survival time and viability of toxoplasma gondii . in addition, the life span of toxoplasma infected animals was investigated. methods: sixty adult male balb/c mice were used for in vi...

SUMMARY Tumor-specific transplantation antigen (TSTA) was solu-bilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by Chromatographie separation and electro-phoresis. The antigen responsible for tumor rejection activ ity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophore...

The soluble and insoluble (particle-bound) tyrosinase (EC 1.10.3.1) present in extracts of Harding-Passey, B-16, and Cloudman S-91 mouse melanoma was examined by means of differential solubility, continuous-flow paper electrophoresis and acrylamide-gel electrophoresis. Two soluble tyrosinases (T1 and T2) were found to exist as distinct entities in each type of melanoma examined. The rela tive c...

Electrophoretic mobility shift assay (EMSA) is widely used in the study of sequence-specific DNA-binding proteins, including transcription factors and mismatch binding proteins. We have established a non-radioisotope-based protocol for EMSA that features an automated DNA sequencer with an infrared fluorescent dye (IRDye) detection unit. Our modification of the elec- trophoresis unit, which incl...

Enzyme with deamidase activity was found in the germinating wheat seeds. The activity appeared in the seeds after 24 hours of germination and reached its maximum value after 48 hours and then declined rapidly. Protein deamidase from germinating wheat seeds was further purified by salting out with phosphate salts and by subsequent chromatography on gel filtration and DEAE-cellulose column chroma...

One of the goals of biology for the next fifty years is to understand how cells work. This fundamentally requires a diverse set of approaches for performing measurements on cells in order to extract information from them. Manipulating the physical location and organization of cells or other biologically important particles is an important part in this endeavor. Apart from the fact that cell fun...