phylogenetic analysis has shown that escherichia coli is composed of four main phylogenetic groups (a, b1, b2 and d). characterization of phylogenetic groups is of clinical interest, as group a and b1 generally associated with commensals, whereas most enteropathogenic isolates are assigned to group d, and group b2 is associated with extra-intestinal pathotype. one hundred e. coli strains recove...

BACKGROUND In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycop...

Abstract. Detection of toxigenic Vibrio cholerae O1/O139 in aquatic environment is difficult to achieve using the culture method. For direct detection of viable toxigenic V. cholerae in aquatic environment, we developed a triplex reverse transcription (RT)-PCR, targeting genes for the outer membrane protein (ompW), cholera toxin A (ctxA) and toxin-coregulated pilli (tcpA) and compared the assay...

escherichia coli (e. coli) is a normal inhabitant of the gastrointestinal tract of vertebrates, including humans. phylogenetic analysis has shown that e. coli is composed of four main phylogenetic groups (a, b1, b2 and d). group a and b1 are generally associated with commensals, whereas group b2 is associated with extra-intestinal pathotypes. most enteropathogenic isolates, however, are assigne...

cholera outbreak is more common in developing countries. the causative agent of the disease is vibrio cholerae strains o1 and o139. traditional diagnostic testing for vibrio is not always reliable, because vibrio can enter a viable but non cultivable state. therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       in this investigation, a ...

Macrolide treatment failure in syphilis patients is associated with a single point mutation (either A2058G or A2059G) in both copies of the 23S rRNA gene in Treponema pallidum strains. The conventional method for the detection of both point mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and time-consuming. We initially developed a TaqMan-based real-tim...

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       In this investigation, a...

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. In this investigation, a triple...

Klebsiella pneumoniae, Corynebacterium kutscheri, and Streptococcus pneumoniae are important pathogens that cause respiratory infections in laboratory rodents. In this study, we used species-specific triplex PCR analysis to directly detect three common bacterial pathogens associated with respiratory diseases. Specific targets were amplified with conventional PCR using the tyrB gene from K. pneu...