wych elm (ulmus glabra hudson) is one of the forest species in hyrcanian forests of iran and considered as threatened species due to its narrow ecological range, over-exploitation, as well as dutch elm disease. in order to evaluate the possibility of cryopreservation of ulmus glabra seeds for long-term preservation, seeds were collected from natural habitats and three pre-treatment methods incl...

Plant shoot tips do not survive exposure to liquid nitrogen temperatures without cryoprotective treatments. Some cryoprotectant solutions, such as plant vitrification solution 2 (PVS2), dehydrate cells and decrease lethal ice formation, but the extent of dehydration and the effect on water freezing properties are not known. We examined the effect of a PVS2 cryoprotection protocol on the water c...

Heterogeneity in morphology, physiology and cellular chemistry of plant tissues can compromise successful cryoprotection and cryopreservation. Cryoprotection is a function of exposure time × temperature × permeability for the chosen protectant and diffusion pathway length, as determined by specimen geometry, to provide sufficient dehydration whilst avoiding excessive chemical toxicity. We have ...

Many plant species can be cryopreserved by treating shoot tips with complex cryoprotectant solutions before rapidly cooling them to liquid nitrogen temperatures. Plant vitrification solution 2 (PVS2), a commonly selected cryoprotectant, can be lethal with extended exposure times. To determine potentially toxic combinations, we have exposed mint shoot tips to one-, two-, three-, and four-compone...

Abstract The objective of this study was to evaluate the efficiency cryoprotective solution (PVS2) combined with phloroglucinol for cryopreservation seeds two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds had 91.03% initial viability 91.99% germination. treatment PVS2 at 0 °C 1% 60 min returned 93.79% 91.01% germination after recovery from LN, consequently resulting in faster...

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. ...