نتایج جستجو برای: transplastomic

تعداد نتایج: 160  

Journal: :Genome Biology 2001

2013
Dheeraj Verma Shuangxia Jin Anderson Kanagaraj Nameirakpam D. Singh Jaiyanth Daniel Pappachan E. Kolattukudy Michael Miller Henry Daniell

In order to produce low-cost biomass hydrolyzing enzymes, transplastomic lines were generated that expressed cutinase or swollenin within chloroplasts. While swollenin expressing plants were homoplasmic, cutinase transplastomic lines remained heteroplasmic. Both transplastomic lines showed interesting modifications in their phenotype, chloroplast structure, and functions. Ultrastructural analys...

Plastid engineering gives numerous benefits for the next generation of transgenic technology, consisting of the convenient use of transgene stacking and the production of high expression levels of recombinant proteins. Designed ankyrin repeat proteins (DARPin) are relatively small non-immunoglobulin scaffold proteins that bind to their specific target with high affinity. The G3 is a type of DAR...

Journal: :Plant physiology 2008
Anna E Sheppard Michael A Ayliffe Laura Blatch Anil Day Sven K Delaney Norfarhana Khairul-Fahmy Yuan Li Panagiotis Madesis Anthony J Pryor Jeremy N Timmis

In eukaryotes, many genes were transferred to the nucleus from prokaryotic ancestors of the cytoplasmic organelles during endosymbiotic evolution. In plants, the transfer of genetic material from the plastid (chloroplast) and mitochondrion to the nucleus is a continuing process. The cellular location of a kanamycin resistance gene tailored for nuclear expression (35SneoSTLS2) was monitored in t...

Journal: :Plant & cell physiology 2006
Hiroshi Yamamoto Hideki Kato Yuki Shinzaki Sayaka Horiguchi Toshiharu Shikanai Toshiharu Hase Tsuyoshi Endo Minori Nishioka Amane Makino Ken-Ichi Tomizawa Chikahiro Miyake

We tested the hypothesis that ferredoxin (Fd) limits the activity of cyclic electron flow around PSI (CEF-PSI) in vivo and that the relief of this limitation promotes the non-photochemical quenching (NPQ) of Chl fluorescence. In transplastomic tobacco (Nicotiana tabacum cv Xanthi) expressing Fd from Arabidopsis (Arabidopsis thaliana) in its chloroplasts, the minimum yield (F(o)) of Chl fluoresc...

2013
Igor Kolotilin Angelo Kaldis Eridan Orlando Pereira Serge Laberge Rima Menassa

BACKGROUND Chloroplast transformation in tobacco has been used extensively to produce recombinant proteins and enzymes. Chloroplast expression cassettes can be designed with different configurations of the cis-acting elements that govern foreign gene expression. With the aim to optimize production of recombinant hemicellulases in transplastomic tobacco, we developed a set of cassettes that inco...

Journal: :Plant physiology 2001
X H Zhang J E Brotherton J M Widholm A R Portis

Anthranilate synthase (AS), the control enzyme of the tryptophan (Trp) biosynthetic pathway, is encoded by nuclear genes, but is transported into the plastids. A tobacco (Nicotiana tabacum) cDNA (ASA2) encoding a feedback-insensitive tobacco AS alpha-subunit was transformed into two different sites of the tobacco plastid genome through site-specific insertion to obtain transplastomic plants wit...

Journal: :Iranian biomedical journal 2013
Mehdi Sharifi Tabar Ali Akbar Habashi Hamid Rajabi Memari

BACKGROUND Human granulocyte colony-stimulating factor (hG-CSF) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. Transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other ad...

Journal: :Environmental biosafety research 2007
Nicole Brinkmann Christoph C Tebbe

Cultivation-independent analyses were carried out to compare the bacterial community structure found in the rhizospheres of a transplastomic tobacco plant carrying the antibiotic resistance marker-gene aadA and its non-engineered parental line. PCR- and reverse transcriptase PCR-amplifications of 16S rRNA and their corresponding genes were carried out with primers targeting the domain Bacteria....

2007
Nicole BRINKMANN

Cultivation-independent analyses were carried out to compare the bacterial community structure found in the rhizospheres of a transplastomic tobacco plant carrying the antibiotic resistance marker-gene aadA and its nonengineered parental line. PCRand reverse transcriptase PCR-amplifications of 16S rRNA and their corresponding genes were carried out with primers targeting the domain Bacteria. Th...

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