during 2004-2007, a field survey was conducted in different vineyards to determine the fungal pathogens associated with grapevine decline in different provinces of iran including fars,hamedan,isfahan, and kohgiluyeh and boirahmad. twenty-seven isolates of botryosphaeriaceae were recovered from vines showing decline and dieback symptoms only in fars province. based on morphological and cultural ...

Bacterial growth significantly depends on protein synthesis catalyzed by ribosome. Ribosome translation elongation speed is a key factor determining the bacterial protein synthesis rate. However, existing methods for determining translation elongation speed have limited applications. Here we developed a simple and convenient method for measuring bacterial translation elongation speed based on L...

Regulation of translation elongation, membrane insertion, and assembly of the chloroplast-encoded D1 protein of photosystem II (PSII) was studied using a chloroplast translation system in organello. Translation elongation of D1 protein was found to be regulated by (1) a redox component that can be activated not only by photosynthetic electron transfer but also by reduction with DTT; (2) the tra...

Codon usage biases are found in all eukaryotic and prokaryotic genomes and have been proposed to regulate different aspects of translation process. Codon optimality has been shown to regulate translation elongation speed in fungal systems, but its effect on translation elongation speed in animal systems is not clear. In this study, we used a Drosophila cell-free translation system to directly c...

background: elongation factor-1, a conserved nuclear protein coding gene was used to identify iranian sandfly species. the phlebotomine sandfies are the vectors of the parasitic protozoan leishmania, the causative agents of leishmaniasis, in iran. methods: seven sets of primers were tried. pcr amplification of elongation factor-1 was successfully achieved for all 14 species of iranian sandfli...