نتایج جستجو برای: tmof

تعداد نتایج: 18  

2011
YS Lau S Sulaiman H Othman

BACKGROUND Trypsin Modulating Oostatic Factor (TMOF) terminates trypsin biosynthesis in adult and larval mosquito gut. It will inhibit the growth and development of mosquito larvae feeding on it resulting in death by starvation. The objective of this study is to determine the effective dose of Pichia-TMOF and the combination of Pichia-TMOF with Bacillus thuringiensis (Bt) as larvicide on Ae. ae...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2006
Dov Borovsky Shailaja Rabindran William O Dawson Charles A Powell Donna A Iannotti Timothy J Morris Jeffry Shabanowitz Donald F Hunt Hendrik L DeBondt Arnold DeLoof

We report the engineering of the surface of the tobacco mosaic virus (TMV) virion with a mosquito decapeptide hormone, trypsin-modulating oostatic factor (TMOF). The TMV coat protein (CP) was fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expression of CP and chimeric CP-TMOF (20:1 ratio) that were coassembled into virus particles in infected Nicotian...

2008
Dov BOROVSKY Ralf NAUEN D. Borovsky R. Nauen

The three-dimensional conformation of Aea-TMOF (Aedes aegypti Trypsin Modulating Oostatic Factor), a decapeptide (YDPAPPPPPP) isolated from mosquito ovaries that inhibits the translation of many trypsin-like serine proteases, e.g. in mosquitoes, flies and lepidopterans, was used as a model for the synthesis of 10 aromatic and aliphatic organic acid and ester analogues. The organic TMOF analogue...

2010
Dov Borovsky Vadim Khasdan Sabine Nauwelaers Clara Theunis Arieh Zaritsky

Starved first instar Aedes aegypti larvae were 35-fold more sensitive to Bacillus thuringiensis subsp. israelensis (Bti) toxins than fed larvae. Feeding larvae Pichia pastoris yeast cells expressing tmfA (synthetic gene coding for the Trypsin Modulating Oostatic Factor of Ae. aegypti) together with Escherichia coli cells expressing Bti toxin genes (cry4Aa, cry11Aa, cyt1Aa and p20) indicate that...

Journal: :journal of arthropod-borne diseases 0
ys lau department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia s sulaiman department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia h othman department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia

background: trypsin modulating oostatic factor (tmof) terminates trypsin biosynthesis in adult and larval mos­quito gut. it will inhibit the growth and development of mosquito larvae feeding on it resulting in death by starvation. the objective of this study is to determine the effective dose of pichia-tmof and the combination of pichia-tmof with bacillus thuringiensi s ( bt) as larvicide on ae...

Journal: :Journal of bacteriology 1992
K M Yen M R Karl

Five genes, tmoABCDE, encoding toluene-4-monooxygenase (T4MO) were previously mapped to a 3.6-kb region of a 10.2-kb SacI DNA fragment isolated from Pseudomonas mendocina KR1 (K.-M. Yen, M. R. Karl, L. M. Blatt, M. J. Simon, R. B. Winter, P. R. Fausset, H. S. Lu, A. A. Harcourt, and K. K. Chen, J. Bacteriol. 173:5315-5327, 1991). In this report, we describe the identification and characterizati...

Journal: :Insect biochemistry and molecular biology 2001
W Zhu A Vandingenen R Huybrechts G Baggerman A De Loof C P Poulos A Velentza M Breuer

The unblocked hexapeptidic Trypsin Modulating Oostatic Factor of the fleshfly, an inhibitor of both trypsin and ecdysone biosynthesis, resists very well proteolytic breakdown by enzymes present in the lumen of the gut of previtellogenic fleshflies. However, when incubated in hemolymph of adult flies, females and males, its half-life time is a mere 0.5 min. In hemolymph of last instar larvae, th...

Journal: :Insect Biochemistry and Molecular Biology 2010

2014
Szymon Chowański Milena Kudlewska Paweł Marciniak Grzegorz Rosiński

Synthetic insecticides are very efficient in insect control but can be harmful for the environment and health. They cause disturbances in ecosystem functioning, are toxic for a wide range of non-target organisms, and have a high tendency to accumulate in the environment. Because of this, some alternatives are being sought. A good solution seems to use biologically active peptides like peptide h...

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