The regression model for a two-segments titration curve with a break-point at the end-point is analyzed. Both linear and nonlinear shapes of the titration curve segments are treated. An effective and simple method discriminates which of two segments is linear or curved. The point and interval estimates of the end-point are calculated by the nonlinear least squares of curve fitting technique. Th...

A new on-line titrator for pH control has been developed. The on-line identification method generates an estimate of the inverse titration curve as a continuous smooth function eliminating the need for selection of the important break points of the titration curve. The time required to obtain the titration curve in the spanned pH range is approximately twice the settling time of the titrator an...

Principal component analysis (PCA) technique is used for the precise determination of pK values from titration curves of amphoteric molecules, such as proteins and amino acids. A regression model is developed based on the effective charge of amphoteric molecules. Partial domain method, a new algorithm, is introduced to minimize or remove errors in extracted pK values from experimental (titratio...

When individual titratable sites in a molecule interact with each other, their pH titration can be considerably more complex than that of an independent site described by the classical Henderson-Hasselbalch equation. We propose a novel framework that decomposes any complex titration behavior into simple standard components. The approach maps the set of N interacting sites in the molecule onto a...

Titration of a trichloracetic acid filtrate of serum with EDTA is carried out at pH 9.8 using an excess of Calmagite indicator. Successive photometric readings of percentage transmission, made with an EEL titrator, are plotted against the volume of EDTA added. There is very little change at first while calcium is being complexed; the reading then decreases rapidly while magnesium is being titra...