vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...

background and aims: the causal agents of viral lettuce big vein disease are two viruse, lettuce big vein associated virus (varicosavirus) and mirafiori lettuce virus (ophiovirus). these viruses have coat proteins of similar size but have different morphologies and serologically unrelated.the purpose of this study was to distinguish and detect lbvav and milv in lettuce fields in tehran province...

Real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-PCR) has been transformed from an experimental technology into a mainstream scientific tool for the detection of RNA. This is because of several factors: 1) it is a homogeneous assay, which eliminates the requirement for post-PCR processing; 2) it has a wide dynamic range; 3) there is little interassay variation; ...

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...

conclusions: this study indicates that rsv is a substantial cause of respiratory tract infection in infants less than 2 years old. rt-pcr offers a rapid method for detection of rsv in hospitalized children with lrti. results: during the study period, 9 children had a positive result for rsv infection. the median age of these patients was 10 months. bronchiolitis was the clinical diagnosis of pa...

background: acute promyelocytic leukemia (apl) is a subclass of acute myeloid leukemia. the chromosomal aberration in 95% of apl cases is t(15; 17) (q22; q21), which prevents cell differentiation. characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. the goal of this study was to develop a new and powerful flow- fish technique to detect the l...

background: the aim of the current study was to assess the mrna levels of two mitochondria-related genes, including nuclear-encoded nrf1 (nuclear respiratory factor 1), mitochondrial transcription factor a (tfam), and mitochondrial-encoded cytochrome c oxidase subunit 1 (mt-co1) genes in various stages of the human oocyte maturation. methods: oocytes were obtained from nine infertile women with...