results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

background: the wilms tumor 1 (wt1) gene is originally defined as a tumor suppressor gene and a transcription factor that overexpressed in leukemic cells. it is highly expressed in more than 80% of acute myeloid leukemia (aml) patients, both in bone marrow (bm) and in peripheral blood (pb), and it is used as a powerful and independent marker of minimal residual disease (mrd);we have determined ...

vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

conclusions this study showed that cccdna can be detected in the plasma specimen of 17.9% of iranian treatment-naive patients with chronic hepatitis b infection. therefore, designing prospective studies focusing on the detection of cccdna in these patients would provide more information. results the mean (sd) age of the patients was 41.1 ± 12.4 years (range, 20 - 62 years). from a total of 106 ...

the ataxia telangiectasia mutated gene (atm), candidate for breast cancer susceptibility gene, encode a 350-kda protein belongs to the core components of dna-damage response machinery. female at carriers have at least 5-fold increase risk for breast cancer. reduction in atm expression is shown in multiple studies in breast tissues. we aimed to perform a research to measure the atm mrna expressi...