To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using euka...

Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain...

the main objective of this study was to purify and characterize an esterase from shewanella sp f88. the enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and q-sepharore chromatography. molecular weight of the enzyme was 62.3 kda according to sds-page data. the enzyme showed an optimum activity at ph 6.5 and 58 ˚c. evo...

background: screening is a routine procedure for isolation of microorganisms which are able to produce special metabolites. purified thermostable α-amylase from bacterial sources is widely used in different industries. in this study we analyzed samples collected from three different hot springs in iran to detect any strains capable of producing thermostable α-amylase. materials and methods: hot...

A total of 520 clinical and environmental isolates of the family Micrococcaceae that fermented glucose anaerobically were tested for their ability to produce coagulase, thermostable nuclease, and deoxyribonuclease. Of these, 450 isolates coagulated rabbit plasma, produced thermostable nuclease, and were identified as Staphylococcus aureus, 447 of which produced a 3+ to 4+ clot. The remaining th...

A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold better than other thermostable DNA polymerases. Since the enzyme also catalyzes pyrophosphorolysis at dideoxy termini, a thermostable inorganic py...

Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure...

Thermostable composite materials based on solid magnesium phosphate and calcium phosphate, as well hybrid binders have been developed investigated. Thermal phase transformations of the composites studied. Strength characteristics determined in temperature range 20–1000 °C. It is shown that obtained high strength properties (compressive reaches 120–130 MPa) are characterised by thermal stability...

Out of various industrial enzymes, thermostable βglucosidases are of great interest, due to their utility in conversion of lingocellulosic material into bioethanol. Determination of the factors responsible for thermostability in β-glucosidases by sequence and structure comparison will be useful in identification, isolation and design of novel thermostable βglucosidases. In the present study the...

background: pectinases are pectin degrading class of enzymes including polygalacturonase (pg), polymethyl galacturonase (pmg), pectate lyase (pel), and pectin esterase (pe) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices.objectives: a highly methylated pectin degrading bacterium from soil covered with fruit was...