the activated sludge process is the most widely used biological wastewater treatment for both domestic and industrial plants in the world. one of the drawbacks of conventional activated sludge (cas) processes is high sludge production. excess sludge treatment and disposal currently represents a rising challenge for wastewater treatment plants (wwtps) due to economic, environmental and regulatio...

Antifreeze protein (AFP) types, I, II and III were tested for their ability to protect red blood cells from lysis during warming, after cryopreservation in hydroxyethyl starch. All three types reduced hemolysis to 25% of control values at similar micromolar concentrations but enhanced lysis as the AFP concentration approached millimolar levels. Site-directed mutants of type III AFP with differe...

The changes in bacteriolytic and proteolytic activities of skin cathepsins L and B were studied Ž . in thermal-stressed European eel Anguilla anguilla . When fish were kept in 108C water, all activities increased to reach maximum levels at Day 8 with varying increasing rates of 3.0 Ž . Ž . Ž . proteolysis , 4.2 lysis of FlaÕobacterium columnare , 5.7 lysis of Vibrio anguillarum and 7.8 Ž . lysi...

Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the sample preparation process is cell lysis. In this work, a simple microfluidic thermal lysis device is used to quickly release intracellular nucleic ...

Article history: Received 25 July 2016 Received in revised form 19 September 2016 Accepted 30 October 2016 Available online xxxx Soluble microbial products (SMP) and extracellular polymeric substances (EPS) produced by photoautotrophic cyanobacteria become substrates for heterotrophic bacteria in photobioreactors (PBRs). Understanding the roles of SMP and EPS depends on reliable extraction and ...

Animal body temperature (Tbody) varies over daily and annual cycles, affecting multiple aspects of biological performance in both endothermic and ectothermic animals. Yet a comprehensive comparison of thermal performance among animals varying in Tbody (mean and variance) and heat production is lacking. Thus, we examined the thermal sensitivity of immune function (a crucial fitness determinant) ...

Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby mak...

Whole blood was heated for twenty minutes at 40 degrees C., 45 degrees C., 50 degrees C., and 55 degrees C. Changes in the osmotic resistance of the heat-exposed cells were then determined. The values obtained were plotted as lysis increments. This treatment revealed groups of cells of varying heat sensitivity. After heating to 50 degrees C., cell groups were therefore prepared by partitioning ...

Thermal hydrolysis pre-treatment of sludge for anaerobic digestion increases biogas production, enhances volatile solids reduction, and improves dewaterability of digested sludge. This study reports the performance of a thermochemical hydrolysis process (TCHP) that utilizes heat (60°C70 °C) and sodium hydroxide for cell lysis. The objective of this study is to evaluate the impacts of implementi...

Recombinant plasminogen activator (reteplase) is a third generation thrombolytic agent which has been used on coronary artery thrombosis and acute myocardial infarction. Clot lysis assay is usually considered as a unique method to evaluate biological activity of reteplase. In this study biological activity of reteplase was determined by APTT (activated partial thromboplastin time) lysis method....