dna polymerase gene from thermus aquaticus strain yt1 was amplified using venttm dna po-lymerase and cloned under the control of x.pr promoter and expression was induced by a shift in tern perature. the culture was then sonicated, and after centrifugation the lysate was treated with poly ethyleneimine followed by a salting-out step. finally the protein was precipitated with ammonium sulfate and...

This paper presents a summary of fundamental principles and applications of modulated induction thermal plasmas that have been developed by the author’s group. The modulated induction thermal plasma is established using a coil current that is modulated to the order of several hundreds of amperes at a millisecond time scale. Such a large current modulation enables the creation of a large disturb...

Original scientific paper The temperature distribution over the cross-section of an induction motor is calculated at the steady state and transient condition. In order to develop smaller and more efficient electric motors, there is a necessity to carry out more thermal analysis in parallel with the traditional electromagnetic design. It has been found that attention to the thermal design can be...

This work investigated the degrees to which energy use in laboratory buildings may be influenced by interior space planning and/or the ways space is used. The potential influences of typical open, mixed and closed plan layouts and their space utilisation densities/intensities were investigated on a good-practice base case using the TAS, Lightscape and Excel software packages. The peak winter re...

Introduction: Oxidative stress is an important factor in the induction of diabetes complications especially peripheral neuropathy and hyperalgesia, and it has been proven that regular aerobic exercise and vitamin E have antioxidant effects. Therefore, this study was designed to examine effects of regular aerobic exercise and vitamin E on thermal pain threshold in streptozocin-induced diabeti...

Induction heating furnaces are employed for vacuum distillation process to recover and consolidate heavy metals after electrorefining operation. Induction heating furnaces of suitable heating rates are required to be developed for this purpose. Hence it is planned to set up a mock-up induction furnace which will simulate the conditions to be realized in actual induction heated vacuum distillati...

Sporadic diploid gynogenetic fry resulted from fertilization with y-irradiated sperm. Their frequency increased appreciably when thermal shock treatments, beginning during the first hour of development (―0.4 OC lasting 6 hrs 45 min ; 26-30 °C lasting 10 min), were used. When the eggs were fertilized with normal sperm, a good triploidization rate was induced by means of heat shocks (27-30...

BACKGROUND AND AIMS Physical dormancy (PY)-break in some annual plant species is a two-step process controlled by two different temperature and/or moisture regimes. The thermal time model has been used to quantify PY-break in several species of Fabaceae, but not to describe stepwise PY-break. The primary aims of this study were to quantify the thermal requirement for sensitivity induction by de...

A hybrid thermal model for accurate estimation of thermal condition of cagerotor induction motors under non-standard supply systems has been presented. The developed thermal model is a combination of lumped and distributed thermal parameters which are available from motor dimensions and other physical constants. The thermal condition of the motor under non-standard supply systems such as unbala...