In complex task domains, such as games, students may exceed their teachers. Such tasks afford diverse means to tradeoff one type of performance for another, combining task elements in novel ways to yield method variations and strategy discoveries that, if mastered, might produce large or small leaps in performance. For the researcher interested in the development of extreme expertise in the wil...

In this paper, the purification of the human recombinant protein expressed in E. coli using the GST Gene Fusion System, by applying various methods of bacterial lysis: sonication, freeze/thaw and beadbeating, is presented. The study was an attempt to compare the properties of the proteins obtained by the sonication method, recommended by manufacturers but inaccessible for many researchers, with...

This investigation examined frozen cycle outcomes with the transfer of a single thawed blastocyst. It also aimed to determine if the suggested model could aid in achieving a better understanding of factors that influence success with frozen blastocyst transfer. A retrospective analysis was conducted of single frozen embryo transfer cycles carried out at the Cleveland Clinic Fertility Centre in ...

Meniscus allografting has provided relief of meniscal injuries that were previously thought irreparable. However, meniscus allograft tissue remains limited and a significant problem. To improve allograft tissue yield, decrease processing costs, and increase graft availability, this study investigated the biomechanical changes of meniscal allograft tissue frozen and thawed multiple times. Specif...

A slow freezing/rapid thawing method for the cryopreservation of human oocytes has been employed using a sodium-depleted culture media. In 53 frozen egg-embryo transfer (FEET) cycles, a 60.4% survival rate post-thaw was obtained and a 62.0% fertilization rate following intracytoplasmic sperm injection. Overall pregnancy rates were 26.4% per thaw attempt, 30.4% per patient, and 32.6% per embryo ...

OBJECTIVES To investigate the viability and differentiation capacity of dental pulp stem cells (DPSCs) isolated from single donors after 2 years of cryopreservation.   METHODS This prospective study was conducted between October 2010 and February 2014 in the Stem Unit, College of Medicine, King Saud University, Riyadh, Saudi Arabia. Seventeen teeth extracted from 11 participants were processe...

Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The p...

in order to investigate the flexural behaviors of rc beams after freeze-thaw cycles, compressive strength test of concrete cubes after 0, 50, 100, 125 freeze-thaw cycles were made, and static flexural experiment of 48 rc beams after 0, 50, 100, 125 freeze-thaw cycles were made. the relationships of relative compressive strength, mass loss rate, relative dynamic elastic modulus and numbers of fr...

Net ecosystem exchange of CO2 was measured at a temperate peatland in southeastern New Hampshire. Classified as a mineral-poor fen owing to deep, water-logged peats that are influenced to a limited extent by groundwater, the ecosystem is dominated by plants such as sedges (Carex spp.) and evergreen shrubs. Ten automatic chambers measured fluxes every 3 h by sampling changes in headspace concent...

The active layer is the top layer of permafrost soils that thaws during the summer season due to increased ambient temperatures and solar radiation inputs. This layer is important because almost all biological activity takes place there during the summer. The depth of active layer thaw is influenced by climatic conditions. Vegetation has also been found to have a strong impact on active layer t...