The TGFb receptors signal through phosphorylation and nuclear translocation of SMAD2/3. SMAD7, a transcriptional target of TGFb signals, negatively regulates the TGFb pathway by recruiting E3 ubiquitin ligases and targeting TGFb receptors for ubiquitin-mediated degradation. In this report, we identify a deubiquitylating enzyme USP11 as an interactor of SMAD7. USP11 enhances TGFb signalling and ...

The TGFb receptors signal through phosphorylation and nuclear translocation of SMAD2/3. SMAD7, a transcriptional target of TGFb signals, negatively regulates the TGFb pathway by recruiting E3 ubiquitin ligases and targeting TGFb receptors for ubiquitin-mediated degradation. In this report, we identify a deubiquitylating enzyme USP11 as an interactor of SMAD7. USP11 enhances TGFb signalling and ...

The TGFb receptors signal through phosphorylation and nuclear translocation of SMAD2/3. SMAD7, a transcriptional target of TGFb signals, negatively regulates the TGFb pathway by recruiting E3 ubiquitin ligases and targeting TGFb receptors for ubiquitin-mediated degradation. In this report, we identify a deubiquitylating enzyme USP11 as an interactor of SMAD7. USP11 enhances TGFb signalling and ...

The TGFb receptors signal through phosphorylation and nuclear translocation of SMAD2/3. SMAD7, a transcriptional target of TGFb signals, negatively regulates the TGFb pathway by recruiting E3 ubiquitin ligases and targeting TGFb receptors for ubiquitin-mediated degradation. In this report, we identify a deubiquitylating enzyme USP11 as an interactor of SMAD7. USP11 enhances TGFb signalling and ...

TGFb inhibits adipocyte differentiation, yet is expressed by adipocytes. The function of TGFb in adipogenesis, and its mechanism of action, is unknown. To address the role of TGFb signaling in adipocyte differentiation, we characterized the expression of the TGFb receptors, and the Smads which transmit or inhibit TGFb signals, during adipogenesis in 3T3-F442A cells. We found that the cell-surfa...

Transforming growth factor-beta (TGFb) is a secreted polypeptide that plays essential roles in cellular development and homeostasis. Although mechanisms of TGFb-induced responses have been characterized, our understanding of TGFb signaling remains incomplete. Here, we uncover a novel function for the protein kinase NDR1 (nuclear Dbf2-related 1) in TGFb responses. Using an immunopurification app...

Lysophosphatidic acid (LPA) is a multifunctional intercellular phospholipid mediator present in blood and other biological fluids. In cancer cells, LPA stimulates expression or activity of inflammatory cytokines, angiogenic factors, matrixmetalloproteinases, and other oncogenic proteins. In this study, we showed that LPA upregulated expression of the cyclin-dependent kinase inhibitor p21 in TGF...

Transforming growth factorb (TGFb ) is secreted by many cell types as part of a large latent complex composed of three subunits: TGFb , the TGFb propeptide, and the latent TGFb binding protein (LTBP). To interact with its cell surface receptors, TGFb must be released from the latent complex by disrupting noncovalent interactions between mature TGFb and its propeptide. Previously, we identified ...

The signalling pathways downstream of the transforming growth factor beta (TGFb) family of cytokines play critical roles in all aspects of cellular homeostasis. The phosphorylation and activation of p38 mitogen-activated protein kinase (MAPK) has been implicated in TGFb-induced epithelial-to-mesenchymal transition and apoptosis. The precise molecular mechanisms by which TGFb cytokines induce th...

Paracrine effect of transforming growth factorb 1 (TGFb 1) on autoimmune insulitis and diabetes was studied by transgenic production of the active form of porcine TGFb 1 (pTGFb 1) in pancreatic islet (islet) a cells in nonobese diabetic (NOD) mice under the control of rat glucagon promoter (RGP) (NOD-RGP-TGFb 1). None of 27 NOD-RGP-TGFb 1 mice developed diabetes by 45 wk of age, in contrast to ...