background: after improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. however, freezing has some harmful effects. melatonin, a pineal gland hormone, has receptors in reproductive sy...

testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. given that extracellular matrix (ecm) facilitates differentiation of spermatogonial stem cells (sscs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ecm on sscs diffe...

background: spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. ubiquitin carboxy-terminal hydrolase l1 (uchl1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (sscs) in testis tissue and during the culture; therefore we undertook this study...

Background: More than a decade work in preservation of male fertility by spermatogonial stem cell (SSC) transplantation in animal models as well as new discoveries about the nature of human SSCs has prompted an investigation into the possibility of an effective clinicalgrade procedure for isolation and cryopreservation of human testicular cells. Materials and Methods: Clinical-grade reagents, v...

objective: spermatogonial stem cells (sscs) are the only cell type that can restore fertility to an infertile recipient following transplantation. much effort has been made to develop a protocol for differentiating isolated sscs in vitro. recently, three-dimensional (3d) culture system has been introduced as an appropriate microenvironment for clonal expansion and differentiation of sscs. this ...

objective: electroporation can be a highly efficient method for introducing the foreign genetic materials into the targeted cells for transient and/or permanent genetic modification. considering the application of this technique as a very efficient method for drug, oligonucleotide, antibody and plasmid delivery for clinical applications and production of transgenic animals, the present study ai...

background: long term alcohol use has been implicated in men with sexual disorders including suppression of testosterone levels as well as testicular morphological changes. objective: this research investigated the ability of telfairia occidentalis (t.o.) to attenuate the damaging effects of alcohol on the testicular parameters. materials and methods: thirty male sprague-dawley rats, 170-190 gr...

Background: Spermatogenesis is under the control of variety of factors, which include mast cells. Any changes in testicular mast cells’ number and distribution resulting in deterioration of comlex regulation and fine-turning of spermatogenesis. Increased number of mast cells and their abnormal spread pattern have been described in the testis with fertility disturbances. Mast cells’ tryptase enz...

introduction: morphology and ultra-structure of myoid and sertoli cells were studied in patients with non-obstructive azoospermia who were admitted to testicular sperm extraction (tese) and intracytoplasmic sperm injection (icsi), by transmission electron microscopy (tem). materials and methods: 12 biopsies of testicular tissues from patients with non-obstructive azoospermia referred to royan i...

abstract diabetes mellitus (dm) is one of the greatest public health threats in modern societies. although during a few years it was suggested that dm had no significant effect in male reproductive function, this view has been challenged in recent years. from a clinical perspective, the evaluation of semen parameters, as well as spermatozoa deoxyribonucleic acid (dna) integrity, are often studi...