interactions of cationic tetrakis (n,n´,n´´,n´´´-tetramethyltetra-3,4-pyridinoporphyrazinatocobalt(ii) (co(tmtppa)) with synthetic polynucleotides, poly(a-t), poly(g-c) and calf thymus dna have been characterized in 5 mm phosphate buffer, ph 7.2, by optical absorption and fluorescence spectroscopy. the appearance of hypochromicity effect and the red shift in uv-vis spectrum of porphyrazine was ...

Synthetic 2-stranded RNA-a helical complex formed by duplexing homopolymers of polyriboinosinic and polyribocytidylic acids (poly I:C)-induced host resistance to foot-and-mouth disease virus when microgram quantities were injected into mice. There was a graded response as shown by titrations of polynucleotide complex or virus. Protection was effective for >48 hours after a single injection of p...

The success of long polynucleotide de novo synthesis is largely dependent on the quality and purity of the oligonucleotides used. Generally, the primary product of any synthesis reaction is directly cloned, and clones with correct products have to be identified. In this study, a novel strategy has been established for removing undesired sequence variants from primary gene synthesis products. Si...

Many substances which do not react with DNA directly are metabolized into important DNA-modifying intermediates. We have devised a method for trapping these intermediates with 14C-labeled nucleosides contained in a synthetic polynucleotide. The polynucleotide structure protects the labeled nucleoside from metabolism; thus, it is unaltered when the polymer is incubated with a drug-metabolizing s...

Erythromycin A released peptidyl-tRNA in the in vitro polypeptide synthesis system with bacterial components programmed by synthetic polynucleotide. This is consistent with our hypothesis that erythromycin A inhibits translocation by preventing proper situation of oligopeptidyl-tRNA in the donor (D) site on ribosomes.

Unambiguous determination of genetic relationships is important to understand mating patterns and population structures. For example, thorough social studies of lekking or cooperatively breeding species require that parentage and other close genetic relationships be determined. Similarly, although extrapair copulations (EPC) have been documented in several bird species (e.g. Birkhead 1987, West...

Bacillus amyloliquefaciens BaM-2 produces large amounts of extracellular enzymes, and the synthesis of these proteins appears to be dependent upon abnormal ribonucleic acid metabolism. A polynucleotide phosphorylase (nucleoside diphosphate:polynucleotide nucleotidyl transferase) was identified, purified, and characterized from this strain. The purification scheme involved cell disruption, phase...

This review is a comprehensive analysis of the progress made so far on the delivery of polynucleotide-based therapeutics to the eye, using synthetic nanocarriers. Attention has been addressed to the capacity of different nanocarriers for the specific delivery of polynucleotides to both, the anterior and posterior segments of the eye, with emphasis on their ability to (i) improve the transport o...