Various methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but some researchers have used the SYBR Green I approach, wh...

introduction: one of the most common problems we confront in orthodontic therapy is periodontal diseases. initial factor which causes these diseases is colonization of anaerobic microorganisms in subgingival plaque. technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. however, it seems necessary to assess va...

Fluorescent monitoring of DNA amplification is the basis of real-time PCR, from which target DNA concentration can be determined from the fractional cycle at which a threshold amount of amplicon DNA is produced. Absolute quantification can be achieved using a standard curve constructed by amplifying known amounts of target DNA. In this study, the mathematics of quantitative PCR are examined in ...

OBJECTIVE We attempted to reveal the changes of the human telomerase reverse transcriptase (hTERT) alternative splicing pattern in gastric carcinogenesis. METHODS Three alternative splicing sites (alpha, beta, gamma) were selected and designed PCR primer. The expression of 8 hTERT alternative splicing variants (ASVs) in normal gastric mucosa, precancerous lesions and gastric cancer were detec...

Purpose: The aim of this study was to determine the effects Echinacoside on cell proliferation, invasion and mRNA expression changes invasion-related genes in PC3 androgen-independent prostate cancer cells.
 Material Methods: effect proliferation cells determined by XTT method. Anti-invasive efficacy achieved using transwell chamber. Total RNA isolation performed Trizol cDNA subsequently s...

Histamine is involved in different physiological and pathological responses, such as immune response, gastric acid secretion or neurotransmission, as either angiogenesis or cancer. Histidine decarboxylase (HDC) catalyzes the formation of histamine from histidine. HDC has been suggested as a new marker for neuroendocrine differentiation, inflammatory pathologies and several leukemia and highly m...

To determine the effect of gelatinase activity of mosquitocidal protein of P. fluorescens Migula strain (VCRC-B426) on larvae and pupae of Culex quinquefasciatus was studied. The results of Enzchek Gelatinase/Collagenase assay revealed that the mosquitocidal metabolite exhibited the gelatinase/collagenase activity and efficiently digested the fluorescein conjugate-DQ gelatin to yield highly flu...