1. The mechanism of sulphite and sulphate permeation into rat liver mitochondria was investigated. 2. Extramitochondrial sulphite and sulphate elicit efflux of intramitochondrial phosphate, malate, succinate and malonate. The sulphate-dependent effluxes and the sulphite-dependent efflux of dicarboxylate anions are inhibited by butylmalonate, phenylsuccinate and mersalyl. Inhibition of the phosp...

a simple, acurate and rapid spectrophotometric method for determination of sulphite thiamine hydrochloride (B1) is described. This used proton transfer reaction between ion with xylenol orange at pH(7.6) to develop red colour adduct that maximum absorption 580 nm. was by bleaching the product (xylenol orange-sulphite) optimum conditions established sulphite. Beer’s law obeyed in concentration r...

NMR and chemometrics was applied to understand the variations in chemical composition of coconut water under different processing. Six processing treatments were applied to coconut water and analyzed: two control (with and without sulphite), and four samples thermally processed at 110°C and 136°C (with and without sulphite). Samples processed at lower temperature and without sulphite presented ...

Electron transfer (ET) in sulphite oxidase (SOx), a heme- and molybdopterin cofactor-containing enzyme, was studied spectroelectrochemically using capillary gold electrode modified with aldrithiol. Direct electron exchange between SOx and the surface of modified gold was observed, with a formal potential of -115 mV vs. Agmid R:AgCl, KCl(sat) at pH 7.0. This value agreed well with that previousl...

The utilization of several inorganic and organic sulphur compounds by three strains of Clostridium perfringens and the production of hydrogen sulphide from these compounds was investigated. Sulphate, sulphite, thiosulphate and sulphide cannot supply sulphur to the organism, neither do they have any effect, when added in non-toxic concentrations, on the growth in media containing utilizable orga...

Autoxidation of sulphite is the first well known chain reaction proceeding through free radicals in solutions. Dark and photochemical autoxidation of sulphite were stated to take place by the same mechanism except in the initial steps. It was found that the formation of sulphite radicals in photoinitiated autoxidation of sulphite which is a metal catalysed reaction as well as the classical ther...

1. The activity of rat liver microsomal sulphite oxidase (EC 1.8.3.1) was increased several-fold on aging of microsomes, on delipidation by extraction with acetone or on solubilization with deoxycholate, suggesting its existence in a cryptic state. 2. In rat liver most of the sulphite oxidase was present in the nuclear fraction and only a small portion in the microsomes. 3. Microsomal sulphite ...

Sodium metabisulphite is used as an antioxidant agent in many pharmaceutical formulations. It is extensively used as a food preservative and disinfectant. It has been demonstrated that sulphite exposure can affect some organs. Curcumin, the main element of Curcuma longa, has been identified to have multiple protective properties. The present study extends the earlier works to quantitative evalu...

the present work reports a kinetic spectrophotometric method for the determination of traceamounts of sulphite that is based on inhibitory effect of sulphite on the oxidation of methyleneblue (mb) in acidic media. the reaction is followed spectrophotometrically by monitoring thedecrease in absorbance of mb at 664 nm with a fixed time of 0.5-4.5 minutes. it was found thatsulphite exert a strong ...

The present work reports a kinetic spectrophotometric method for the determination of traceamounts of sulphite that is based on inhibitory effect of sulphite on the oxidation of Methyleneblue (MB) in acidic media. The reaction is followed spectrophotometrically by monitoring thedecrease in absorbance of MB at 664 nm with a fixed time of 0.5-4.5 minutes. It was found thatsulphite exert a strong ...