conclusions allicin vapor, or some decomposition product of allicin, causes inhibition of bacterial growth and biofilm formation. parallel enzymatic studies confirmed this inhibitory effect and suggest that sulfhydryl enzymes are involved in biofilm formation in the strain studied. results an inhibition effect was observed both in the bacterial cultures and the enzymatic solutions, and the exte...

ceruloplasmin (cp) is a human plasma protein with multiple physiological functions including ferroxidase and oxidase activities. deficiency or inhibition of the enzyme may lead to some abnormalities, including iron deposition in several tissues and causes various pathological conditions in the body. direct interaction of cadmium (cd), a widespread, highly toxic environmental pollutant, with hum...

The results of previous work have indicated (2, 3) that one sulfhydryl group of microsomal cytochrome reductase is essential for the interaction of nucleotides with this enzyme. These results, however, do not rule out the possibility that there are other reactive sulfhydryl groups on the native enzyme and that they are involved directly or indirectly in nucleotide-enzyme interactions. In the ex...

1. The mechanism of succinic dehydrogenase action was studied by means of inhibitors. 2. The enzyme is inhibited by a large number of diverse compounds whose only common denominator appears to be their ability to react with SH groups. These compounds include quinonoid structures, sulfhydryl reagents, sulfhydryl compounds, copper, zinc, selenite, and arsenite. 3. In contrast to the above inhibit...

A number of different enzymes are activated by certain reducing agents (e.g., KCN, H&S, cysteine, and reduced glutathione) and inactivated by oxidizing agents (e.g., K3Fe(CN)&, HzOz, and cystine). Under ideal conditions such inactivation is reversible by the addition of reducing agents (for a review of this subject cJ Hellerman (1937, 1939)). These results have been interpreted on the basis of ...

Spinach leaf glyoxylic acid reductase, an enzyme containing two identical or similar subunits, has been shown to have 12 half-cystine residues. Four of these are necessary for catalytic function and can be titrated as free sulfhydryl groups. One mole of cysteinyl residues per mole of enzyme is essential to catalytic activity when evaluated under conditions favoring optimal activity. All free su...

The yellow colored sulfhydryl reagent, iV-(4-dimethylamino-3,!%dinitrophenyl)maleimide, was used in the isolation of the peptide containing the reactive sulfhydryl group of chymopapain. Titration of cyanide-activated chymopapain B with p-chloromercuribenzoate at pH 4.6 indicated that there is a maximum of 1.4 moles of sulfhydryl per mole of the enzyme. Alkylation of half of the p-chloromercurib...

The metal cation requirements of pyruvate, phosphate dikinase from Bacteroides symbiosus have been examined with respect to both the overall reaction and the three partial reactions. The overall reaction and all three partial reactions require a divalent cation (M$‘, Mn’+, or Co’+). Although Mn2+ and Co’+ are very effective activators at low concentrations in two of the three partial reactions,...

cirrhosis is the consequence of chronic liver disease. deleterious effects of oxidative stress on hepatocytes may be reflected in the erythrocyte membrane. naltrexone (ntx) has been shown to attenuate hepatocellular injury in fibrotic animal models. the aim of this study was to investigate the progressive effect of ccl 4 on the liver and whether the improvement of liver cirrhosis can be monitor...