• PfDHPS-A437G is known as one of sulfadoxine-resistant mutations in P. falciparum . PbDHPS-A394G berghei corresponds to PfDHPS-A437G. We generated a transgenic parasite and investigated the developments. grew mice produced oocysts with similar efficiency wild-type. The mutation may not impose fitness costs on during examined stages. Genetic changes conferring drug resistance are generally beli...

Sulfadoxine is predominantly used in combination with pyrimethamine, commonly known as Fansidar, for the treatment of Plasmodium falciparum. This combination is usually less effective against Plasmodium vivax, probably due to the innate refractoriness of parasites to the sulfadoxine component. To investigate this mechanism of resistance by P. vivax to sulfadoxine, we cloned and sequenced the P....

Sulfadoxine/pyrimethamine is recommended for intermittent preventative treatment of malaria during pregnancy. Data from 98 women during pregnancy and 77 after delivery in four African countries were analyzed using nonlinear mixed-effects modeling to characterize the effects of pregnancy, postpartum duration, and other covariates such as body weight and hematocrit on sulfadoxine/pyrimethamine ph...

CONTEXT In malaria-endemic regions, strategies to control malaria during pregnancy rely on case management of malaria illness and anemia, and preventive measures such as insecticide-treated nets and intermittent preventive therapy (IPT). OBJECTIVE To determine the effect of increasing resistance to sulfadoxine-pyrimethamine on the efficacy of IPT during pregnancy in Africa. DATA SOURCES AND...

Sulfadoxine-pyrimethamine has never been recommended for the treatment of Plasmodium vivax malaria as the parasite is intrinsically resistant to pyrimethamine. The combination was introduced as a promising agent to treat Plasmodium falciparum malaria in many countries but was withdrawn after a few years due to development and spread of resistant strains. Presently, sulfadoxine-pyrimethamine is ...

The disposition of sulfadoxine was studied in the presence of pyrimethamine in 18 healthy Thai subjects who had been suffering from falciparum malaria in the 6 months prior to the study, and in 12 Thai patients with acute malaria. The volunteers were administered an oral dose of 500 mg sulfadoxine + 25 mg pyrimethamine (1 Fansidar tablet). They were classified retrospectively as responders (Gro...

background: point mutations in the genes, pfcrt and pfdhfr , have been identified elsewhere as strongly associated with re­sistance to chloroquine (cq) and pyrimethamine, respectively.  methods: using a restriction digestion method, we report the frequency of two important mutations, t76 and asn108, re­spon­sible for conferring resistance to chloroquine and pyrimethamine, respectively, in patie...

New simple sensitive spectrophotometric methods are developed for the estimation of Sulfadoxine (SFD) in pure and pharmaceutical formulations. The first method includes a conversion primary amine to azo-dye by reacting sulfadoxine with sodium nitrite hydrochloric acid followed coupling with4-methoxyphenol alkaline medium obtain stable reddish-orange colored dye at λmax495nm. Concentration range...

Pyrimethamine-sulfadoxine, the first choice for uncomplicated falciparum malaria in Africa, exerts strong selection pressure for resistance because of its slow elimination. It is likely that resistance will emerge rapidly, and there is no widely affordable replacement. Chlorproguanil-dapsone is cheap, rapidly eliminated, more potent than pyrimethamine-sulfadoxine, and could be introduced in the...

OBJECTIVE To measure the efficacy of sulfadoxine-pyrimethamine treatment of falciparum malaria in Malawi from 1998 to 2002, after a change from chloroquine to sulfadoxine-pyrimethamine as first line treatment in that country in 1993. DESIGN Prospective open label drug efficacy study. SETTING Health centre in large peri-urban township adjacent to Blantyre, Malawi. PARTICIPANTS People prese...