results the pellet of the 146s antigen of fmd virus serotype a was developed at a concentration of sucrose 50%. absorbance rate of the foot-and-mouth disease virus serotypes a at wavelengths of 240, 259 and 280 nm was 1.238, 1.573 and 1.157, respectively. moreover, the amount of 146s antigen at the same wavelengths was 163.416, 207.636 and 152.724 μg/ml, respectively. the amount of purified pro...

Soluble sugar accumulation pattern and contents were studied in two Iranian melon cultivars, ‘Suski Sabz’ and ‘Jalali Zard’ (Cucumis melo L. Inodorous). Sucrose, glucose and fructose contents were determined in different mesocarp tissues of developing fruits during days after pollination (DAP). They were characterised by enhanced accumulation of glucose and fructose during early fruit developme...

A crude pea (Pisum sativum L. var. Homesteader) mitochrondrial preparation was divided into two equal parts. One part was layered on a Dextran-40 step gradient, and the other on a sucrose step gradient, and they were centrifuged to obtain different bands of particles. The densities at which the particles banded and the mitochondrial respiratory activities of the particles were determined. Dextr...

Membrane from individual cataractous and normal human lenses has been prepared by discontinuous sucrose gradient centrifugation. A sensitive solid-phase radioimmunoassay has been developed to quantitate the levels of a, 0, and y crystallins associated with membrane obtained from each sucrose gradient interface. The results of this analysis demonstrate significant levels of all three crystallins...

Sucrose uptake by excised developing soybean cotyledons shows a biphasic dependence on sucrose concentration. At concentrations less than about 50 millimolar external sucrose, uptake can be described as a carrier-mediated process, with a K(m) of 8 millimolar. At higher external sucrose concentrations, a linear dependence becomes apparent, which suggests the participation of a nonsaturable compo...

Na+ was found to be essential for the accumulation of sucrose by Vibrio alginolyticus. Sucrose uptake was completely inhibited by the addition of proton conductor at neutral pH, but not at alkaline pH, where the primary electrogenic Na+ pump generates the Na+ electrochemical gradient. We therefore conclude that sucrose transport is driven by the electrochemical potential of Na+ in this organism.

In rat mammary carcinoma, 2 types of cytoplasmic estradiol-binding sites, A and B, are present. The A sites are excluded on Sephadex G-100 chromatography, sediment at 8 S and 4 S in a low-ionic-strength sucrose gradient or at 4 S alone in a high-ionic-strength sucrose gradient, and form an insoluble complex with protamine sulfate. They have a very high affinity for estradiol (Kd ~ 1.2 X IO"10 M...

Clinical isolates from protozoan parasites such as Giardia lamblia are at present practically impossible to culture. By using simple cyst purification methods, we show that Giardia whole genome sequencing of clinical stool samples is possible. Immunomagnetic separation after sucrose gradient flotation gave superior results compared to sucrose gradient flotation alone. The method enables detaile...